Prototyped bioresorbable scaffold as an alternative to dermal matrix

Abstract

In the treatment of a skin wound is necessary to know all the physiological process of wound healing, because there are situations when is necessary to take actions to optimize it. In complex wounds is necessary skin autograft, but, sometimes, there is not the appropriate amount of donor area, or the receptor area is not in conditions to receive the graft. Thus, one alternative is the use of dermal matrix associated with partial skin grafts, which promotes better integration at the receptor site. However, the available products in market are imported and too much expensive. The use of bioabsorbable polymers has been increasing in the medical field since they represent a viable alternative for cases where tissue regeneration is strongly compromised. Another great advantage that the use of these materials can bring is their economic added value, enabling the expansion of this essential procedure. Therefore, the aim of this project is to evaluate the efficiency of the prototyped bioresorbable three-dimensional device PL-co-co-TMC-DLA as dermal matrix associated with partial skin graft in rats as an experimental model for the treatment of cutaneous lesions complex, and to evaluate their biocompatibility. Thereunto, a 1.5 x 1.5 cm cutaneous wound will be excised in the dorsal region of 40 rats. With the Blair's knife, it will be extracted partial skin graft from another part of the dorsum of each animal. As treatment, the wounds will be covered by the scaffold of PL-co-co-TMC-DLA with skin autograft. The control group will be only covered with the autograft. Finishing up periods of 7 and 60 days, the animals will be sacrificed and the integration between the device and bioresorbable dermal graft will be evaluated, as well as the process of tissue repair through histological and histomorphometric analysis.