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Traditional taxonomy and analysis of the 18S rDNA, ITS2 and rbcL sequences of species of the freshwater microalgae family Selenastraceae

Grant number: 13/17457-3
Support Opportunities:Scholarships abroad - Research Internship - Doctorate (Direct)
Effective date (Start): March 01, 2014
Effective date (End): October 31, 2014
Field of knowledge:Biological Sciences - Botany - Plant Taxonomy
Principal Investigator:Armando Augusto Henriques Vieira
Grantee:Thaís Garcia da Silva
Supervisor: Christina Bock
Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
Research place: University of Duisburg-Essen, Germany  
Associated to the scholarship:12/19520-1 - Biodiversity of Selenastraceae (freshwater Chlorococcaceae microalgae): morphological characteristics and sequencing of 18S rDNA as a traditional taxonomic basis for obtaining the DNA barcode, BP.DD


This project proposes the use of traditional methods of taxonomy and analysis of sequences of the 18S rDNA, ITS2 and rbcL of species of freshwater microalgae family Selenastraceae. The main objective is to provide a basis for taxonomic identification of these organisms, whose biodiversity is currently scattered in "complex" Ankistrodesmaceae / Selenastraceae / Chlorellaceae / Oocystaceae, a taxonomically problematic group. For species level resolution, obtaining DNA barcode is a powerful tool, but it does not obviate the practices of traditional taxonomy: morphology, chemotaxonomy, and other collateral information (Hajibabaei et al. 2007). Organisms must be preliminarily entered into a reliable taxonomic system even if, in some groups, it may not be enough to differentiate the species without errors (as is the case of microalgae with their high morphological convergence rates, high incidence of polymorphism and cryptic species). In these cases, the DNA barcode associated with traditional methods of the taxonomy may be the definitive factor in the proper identification of the species. A relatively sound taxonomic base, based on morphological characters, 18S rDNA phylogenetic analysis and specific biochemical profiles is a great starting point for the provision of "vouchers" for obtaining DNA barcodes, but stil need complementary information. In coccoid green algae, the morphological features, although subjective, are extremely useful and necessary as a starting point, and in many cases might be determinants for differentiation (Fawley et al. 2005). However, morphological differences between the species are often only detectable through the cultures, and therefore the species employed in this study will be grown and sampled at various growth stages . (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA SILVA, THAIS GARCIA; BOCK, CHRISTINA; SANT'ANNA, CELIA LEITE; BAGATINI, INESSA LACATIVA; WODNIOK, SABINA; HENRIQUES VIEIRA, ARMANDO AUGUSTO. Selenastraceae (Sphaeropleales, Chlorophyceae): rbcL, 18S rDNA and ITS-2 secondary structure enlightens traditional taxonomy, with description of two new genera, Messastrum gen. nov. and Curvastrum gen. nov.. Fottea, v. 17, n. 1, p. 1-19, . (12/19520-1, 13/17457-3, 11/50054-4, 13/18083-0)
GARCIA, T. S.; BOCK, CHRISTINA; BAGATINI, INESSA LACATIVA; ARCHANJO, NAIARA CAROLINA PEREIRA; SANT'ANNA, CELIA LEITE; VIEIRA, ARMANDO AUGUSTO HENRIQUES. Hidden diversity in Selenastraceae (Sphaeropleales, Chlorophyceae): Molecular phylogeny revealed a nannoplanktonic species, with the description of Viridiparva madeirensis gen. et sp. nov.. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, v. 68, p. 9-pg., . (11/50054-4, 13/17457-3, 12/19520-1, 19/03177-5, 13/18083-0, 16/07089-5)
BAGATINI, INESSA LACATIVA; MECCHERI, FABRICIO SEBASTIANI; PAULSEN, BERIT SMESTED; DA SILVA, THAIS GARCIA; BARSETT, HILDE; HENRIQUES VIEIRA, ARMANDO AUGUSTO. Diversity of monosaccharides and glycosidic linkages on extracellular polysaccharides of the microalga Ankistrodesmus (Chlorophyceae). PHYCOLOGIA, v. N/A, p. 12-pg., . (13/18083-0, 19/03177-5, 13/17457-3, 12/19520-1, 12/00221-4, 11/50054-4)

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