The biomes Cerrado and Atlantic Rain Forest are considered hotspots, threatened in the highest degree. In São Paulo state, despite the intense fragmentation occurred in the last decades, there are still remnants of these important areas. The large-scale deforestation, has serious consequences for the environment, one of the most discussed is global warming. Estimates of forest biomass are extremely important for the climatic effect, since they are directly related to carbon stocks that are used to quantify the greenhouse gases released into the atmosphere in case of a degradation. So currently, carbon sequestration is an environmental services most valued by the market, moving the global economy. Brazil is a country with great potential in the implementation of CDM (Clean Development Mechanism), mainly forest, but still has a lot to develop in this aspect. The percentage of total credits CERs (Certified Emission Reduction) recorded in these projects is expected much lower (3.5%), compared with China (65%) (UNFCCC, 2013), for example. In a forest environment there are various forms of biomass accumulation: above ground, below ground, litter, dead wood and soil. The dead wood contains carbon in woody debris, thick roots, standing trees. The woody debris reduce soil erosion, serve as a reservoir of nutrients, energy and water, influence the development of forest cover, they are habitats for microbial life and animals, and store large amounts of carbon. With so many features, the study of wood debris is of high relevance. The present work aims to develop a methodology for quantifying the volume of dead wood fallen, its biomass and its carbon stock using the theory of line intercept sampling method, testing three sizes of lines at different sampling techniques, the stratified simple systematic and cluster sampling in three vegetation types in the State of São Paulo: Ombrophilous Dense Forest, Seasonal Semideciduous Forest and Cerrado sensu-stricto.
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