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Grant number: 13/09533-1
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): November 01, 2013
Effective date (End): January 20, 2015
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal researcher:Marília Afonso Rabelo Buzalaf
Grantee:Cristiane de Almeida Baldini Cardoso
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


Xylitol, a sugar substitute, has gained attention in the last years due to its antimicrobial, anticaries and remineralizing properties. Thus, effective and safe vehicles are desirable for caries control. Considering that sodium fluoride varnishes are broadly employed in Dentistry to control caries, the present study aims to evaluate the efficacy of a new varnish having xylitol as active principle, in different concentrations, associated or not with fluoride for: 1) in vitro inhibit demineralization and increase remineralization of bovine enamel, 2) on dental enamel de-remineralization in situ and 3) to clinically control active non-cavitated caries lesions (white-spot lesions). For this purpose, in phase 1, the effect of 7 different varnishes (10% Xylitol, 20% Xylitol, 10% Xylitol + 5% NaF, 20% Xylitol + 5% NaF, Duofluoride XII®, Duraphat® and placebo varnish - without xylitol or fluoride) on the inhibition of demineralization and increase of remineralization of sound or artificially demineralized bovine enamel, using an in vitro pH-cycling model will be evaluated (total n = 280 - 20 for each group). In phase 2, 20 will take part in the crossover, split-mouth and double-blind study performed in 4 phases. Four enamel samples will be worn in palatal appliances (2 sound and 2 demineralized), being each type of sample submitted to one treatment: a) Duraphat®, b) xylitol varnish (the one that presents the best result in phase 1), c) 5% NaF xylitol varnish (the one that presents the best result in phase 1) and, d) placebo (without xylitol or fluoride). Finally, in phase 3, 120 6-12-year-old schoolchildren presenting active non-cavitated caries lesions will receive 4 applications with one-week interval, of one of the varnishes evaluated by in situ study. The examination of caries lesions at baseline and at the end of the study will be done using the QLF system (F and lesion area), associated with clinical examination (Nyvad index). Data will be tested for normality and homocedasticity for selection of the appropriated statistical test to be used. The significance level will be set at 5%.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CARDOSO, C. A. B.; CASSIANO, L. P. S.; COSTA, E. N.; SOUZA-E-SILVA, C. M.; MAGALHAES, A. C.; GRIZZO, L. T.; CALDANA, M. L.; BASTOS, J. R. M.; BUZALAF, M. A. R. Effect of xylitol varnishes on remineralization of artificial enamel caries lesions in situ. Journal of Dentistry, v. 50, p. 74-78, JUL 2016. Web of Science Citations: 7.
CARDOSO, C. A. B.; LEVY, F. M.; PERES-BUZALAF, C.; BUZALAF, M. A. R. Dentifrice pH but not consistency may affect fluoride uptake in plaque. Journal of Dentistry, v. 43, n. 2, p. 219-224, FEB 2015. Web of Science Citations: 2.
CARDOSO, C. A. B.; DE CASTILHO, A. R. F.; SALOMAO, P. M. A.; COSTA, E. N.; MAGALHAES, A. C.; BUZALAF, M. A. R. Effect of xylitol varnishes on remineralization of artificial enamel caries lesions in vitro. Journal of Dentistry, v. 42, n. 11, p. 1495-1501, NOV 2014. Web of Science Citations: 12.

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