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Procedures for automated seedling image analysis for vigor evaluation of maize seeds in comparison with other traditional tests

Grant number: 13/11132-5
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2013
Effective date (End): January 31, 2015
Field of knowledge:Agronomical Sciences - Agronomy - Crop Science
Principal researcher:Julio Marcos Filho
Grantee:Danielle Otte Carrara Castan
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Associated research grant:06/57900-0 - Seed imaging analysis in seed technology research, AP.TEM


The role of research in the development of new technologies is essential to increase the efficient evaluation of the seed physiological potential in quality control programs of seed companies. The objectives of this research are to evaluate the physiological potential of maize seeds using automated systems based on seedling image analysis (SVIS® and Vigor-S softwares) in comparison to the information from other accepted methods for vigor evaluation of this species, including the salt solution accelerated aging (SSAA) with NaCl. Seeds of two hybrids represented by six lots each will be used. Evaluations of germination, cold test, traditional accelerated aging, SSAA and field seedling emergence will be performed in three experimental times. For seed vigor evaluation by seedling image analysis three-day-old seedlings will be evaluated by the Seed Vigor Imaging System (SVIS®), developed by Ohio State University/USA and the results will be compared with those from the Automated Analysis of Seed Vigor (Vigor-S), developed by USP/ESALQ and Embrapa Agricultural Instrumentation (CNPDIA). Data will be submitted to analysis of variance in a completely randomized experimental design, except for field seedling emergence, which will be analyzed in randomized blocks. Mean results will be compared by the Tukey's test (p d 0.05). For each evaluation time, data will be separately analyzed for each physiological potential test and hybrid.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CARRARA CASTAN, DANIELLE OTTE; GOMES-JUNIOR, FRANCISCO GUILHIEN; MARCOS-FILHO, JULIO. Vigor-S, a new system for evaluating the physiological potential of maize seeds. Scientia Agricola, v. 75, n. 2, p. 167-172, . (13/11132-5, 06/57900-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
CASTAN, Danielle Otte Carrara. The use of computerized imaging analysis of seedlings to evaluate vigor of corn seed compared to traditional tests. 2015. Master's Dissertation - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.

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