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Effects of strength training on skeletal muscle in mice with cachexia induced cancer.

Grant number: 13/04744-4
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2013
Effective date (End): March 31, 2015
Field of knowledge:Health Sciences - Physical Education
Principal Investigator:Antonio Herbert Lancha Junior
Grantee:Willian das Neves Silva
Host Institution: Escola de Educação Física e Esporte (EEFE). Universidade de São Paulo (USP). São Paulo , SP, Brazil


The objective of this study will be investigate the effects of strength training on survival and skeletal muscle of rats inoculated with Walker 256 tumor. Specifically, we are interested in investigating the effect of exercise on muscle structure and function of animals and the involvement of ubiquitin-proteasome proteolytic system and the involvement of protein synthesis via IGF-1/Akt/Mtor on muscle atrophy in rats with cachexia induced by cancer after strength training. Methods: Two experiments will be performed. The first will seek to verify that strength training increases survival of rats inoculated with walker 256 tumor, for this after the adaptation phase in training equipment, animals will be randomized to form 3 groups, namely : 1) controls (C, n = 5), 2) rats inoculated with Walker 256 tumor (W, n = 15) and 3) rats inoculated with Walker 256 tumor and subjected to strength training (WT, n = 15 ). Experimental groups will pass through the respective interventions until all the animals that developed tumor (WT and W groups) die. In the second experiment, after the adaptation phase, the animals will be randomized to compose 4 experimental groups, namely: 1) healthy rats - control (C, n = 10), 2) healthy mice subjected to strength training (CT, n = 10), 3) rats inoculated with Walker 256 tumor (W, n = 10) and 4) rats inoculated with Walker 256 tumor and subjected to strength training (WT, n = 10). Before tumor inoculation and 14 days after, all experimental groups will perform tests of strength and muscle function and maximal treadmill test. After 16 days of tumor inoculation, the animals will be euthanized and will be removed the muscles soleus and extensor digitorum longus (EDL) for histological and bio molecular analysis, including ex vivo muscle function, proteasome activity, gene expression of Atrogin, MuRF -1, and protein expression of IGF-1, AKT-1, mTOR and p70.

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
W. DAS NEVES; L.F. DE OLIVEIRA; R.P. DA SILVA; C.R.R. ALVES; A.H. LANCHA JR.. Fasting: a major limitation for resistance exercise training effects in rodents. Brazilian Journal of Medical and Biological Research, v. 51, n. 1, . (13/04744-4, 12/13026-5)
DAS NEVES, WILLIAN; RODRIGUES ALVES, CHRISTIAN ROBLES; DE ALMEIDA, NEY ROBSON; RODRIGUES GUIMARAES, FATIMA LUCIA; RAMIRES, PAULO RIZZO; BRUM, PATRICIA CHAKUR; LANCHA, JR., ANTONIO HERBERT. Loss of strength capacity is associated with mortality, but resistance exercise training promotes only modest effects during cachexia progression. Life Sciences, v. 163, p. 11-22, . (13/04744-4, 14/03016-8)
DE CAMPOS-FERRAZ, PATRICIA LOPES; ANDRADE, ISABEL; DAS NEVES, WILLIAN; HANGAI, ISABELA; RODRIGUES ALVES, CHRISTIANO ROBLES; LANCHA, JR., ANTONIO HERBERT. An overview of amines as nutritional supplements to counteract cancer cachexia. JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE, v. 5, n. 2, p. 105-110, . (12/02682-9, 13/04744-4, 12/25240-1, 12/07319-0)
DANTAS, WAGNER SILVA; DAS NEVES, WILLIAN; GIL, SAULO; GRIMALDI BARCELLOS, CRISTIANO ROBERTO; ROCHA, MICHELE PATROCINIO; DE SA-PINTO, ANA LUCIA; ROSCHEL, HAMILTON; GUALANO, BRUNO. Exercise-induced anti-inflammatory effects in overweight/obese women with polycystic ovary syndrome. CYTOKINE, v. 120, p. 66-70, . (12/14650-4, 13/04744-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Willian das Neves. Effects of strength training on skeletal muscle in rats with cachexia-induced cancer. 2016. Master's Dissertation - Universidade de São Paulo (USP). Escola de Educação Física e Esportes (EEFE/BT) São Paulo.

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