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Effects of sperm factors on in vitro embryo production and embryo quality in bovine

Grant number: 13/07940-9
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2013
Effective date (End): May 31, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal researcher:Mayra Elena Ortiz D' Avila Assumpcao
Grantee:Adriano Felipe Perez Siqueira
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil


In vitro production (IVP) in cattle is an important tool for animal reproduction. Despite its commercial and research applications, the results are considered unsatisfying. Sperm factors could reflect on embryo development during IVP. The correlation of seminal quality evaluation methods with fertility prediction has been strongly studied. However, the relation between fertility and seminal quality remains unpredictable, even with the advances in seminal analysis with the employment of specific probes and flow cytometers. These techniques allow analysis of a greater number of sperm cells and seminal parameters simultaneously. The goal of this work is to assess the effects of sperm parameters, such as plasmatic membrane integrity, intact acrosome, high potential of mitochondrial membrane, chromatin structure resistance and sperm DNA integrity, on IVP rates and embryo quality. In this study the effects of these parameters will be evaluated considering the cleavage time and rate, blastocyst rate and the analysis of expression profile of blastocysts, using as target genes some ones that show in literature correlated to embryo quality. This study proposes to clarify the effect and influence of each parameter on the fecundation process and early embryo development. IVP is not only an experimental model, it has economic relevance. The elucidation of the sperm effects could help on the choice of bulls or semen samples for breeding programs using reproductive technologies.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEREZ SIQUEIRA, ADRIANO FELIPE; DE CASTRO, LETICIA SIGNORI; DE ASSIS, PATRICIA MONKEN; BICUDO, LUANA DE CASSIA; MENDES, CAMILLA MOTA; NICHI, MARCILLIO; VISINTIN, JOSE ANTONIO; ORTIZ D'AVILA ASSUMPCAO, MAYRA ELENA. Sperm traits on in vitro production (IVP) of bovine embryos: Too much of anything is good for nothing. PLoS One, v. 13, n. 7, . (13/07940-9, 16/15147-5)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SIQUEIRA, Adriano Felipe Perez. Effect of sperm traits on bovine in vitro production. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.

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