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Transcriptional profile analysis of lignified tissues in Tectona grandis L. f

Grant number: 13/06299-8
Support Opportunities:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): September 01, 2013
Effective date (End): March 31, 2015
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Helaine Carrer
Grantee:Esteban Galeano Gómez
Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil


Teak tree (Tectona grandis L. f.) is a tropical species with great economic interest currently due to its unique wood characteristics such as strength, durability and golden color. However, despite the growth in forestry fields, little is known about its biology, particularly in terms of molecular studies. Since such studies can contribute to understand wood formation, this project aims to identify and characterize genes potentially involved in the formation of lignified tissues through detailed analysis of the T. grandis transcriptome. At first, it will be analyzed the transcriptional profile of teak lignified tissues via RNAseq, subsequently it will be evaluated differential gene expression in those tissues, and finally studies will be conducted to characterize the Cinnamyl alcohol dehydrogenase (CAD) gene family, essential for catalyzing reactions in monolignols formation, final precursors of lignin. Previous results of CAD differential gene expression in teak showed an increased in transcription in branch vascular tissues, followed by trunk tissues, and inside the wood of this tree, tissue with the highest expression were heartwood, sapwood and followed by the inner core. Consequently, all the information will be analyzed with bioinformatics tools to search for differentially expressed genes important in teak wood formation with the data generated in the RNAseq, and then specific primers will be designed to assess differences in the unigenes expression by real-time PCR. The prospect of the project is extensive and aims to have the identification of important unigenes from teak transcriptome of lignified tissues, with emphasis on CAD gene family. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GALEANO, E.; BARROSO, A. A. M.; VASCONCELOS, T. S.; LOPEZ-RUBIO, A.; ALBRECHT, A. J. P.; VICTORIA FILHO, R.; CARRER, H.. EPSPS variability, gene expression, and enzymatic activity in glyphosate-resistant biotypes of Digitaria insularis. Genetics and Molecular Research, v. 15, n. 3, . (12/10371-3, 13/06299-8, 12/10372-0, 10/19294-6)
GALEANO, ESTEBAN; VASCONCELOS, TARCISIO SALES; DE OLIVEIRA, PERLA NOVAIS; CARRER, HELAINE. Physiological and molecular responses to drought stress in teak (Tectona grandis L.f.). PLoS One, v. 14, n. 9, . (13/06299-8, 10/19294-6)
GALEANO, ESTEBAN; VASCONCELOS, TARCISIO SALES; CARRER, HELAINE. Characterization of Cinnamyl Alcohol Dehydrogenase gene family in lignifying tissues of Tectona grandis L.f.. SILVAE GENETICA, v. 67, n. 1, p. 1-11, . (13/06299-8, 10/19294-6)
GALEANO, ESTEBAN; VASCONCELOS, TARCISIO SALES; VIDAL, MABEL; MEJIA-GUERRA, MARIA KATHERINE; CARRER, HELAINE. Large-scale transcriptional profiling of lignified tissues in Tectona grandis. BMC PLANT BIOLOGY, v. 15, . (13/06299-8)
TAMBARUSSI, EVANDRO V.; ROGALSKI, MARCELO; GALEANO, ESTEBAN; BRONDANI, GILVANO EBLING; DE MARTIN, VALENTINA DE FATIMA; DA SILVA, LUCAS AMERICO; CARRER, HELAINE. Efficient and new method for Tectona grandis in vitro regeneration. Crop Breeding and Applied Biotechnology, v. 17, n. 2, p. 124-132, . (08/00046-2, 13/06299-8, 07/05795-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
GÓMEZ, Esteban Galeano. Characterization of genes involved in lignin biosynthesis in Tectona grandis. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.

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