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Protective efficacy of active immunization with the recombinant lipoproteins of Staphylococcus aureus in an experimental model of bacteremia

Grant number: 13/10975-9
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): April 01, 2014
Effective date (End): March 31, 2015
Field of knowledge:Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
Principal researcher:Maria de Lourdes Ribeiro de Souza da Cunha
Grantee:Eliane Patricia Lino Pereira Franchi
Supervisor abroad: Binh An Diep
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Research place: University of California, San Francisco (UCSF), United States  
Associated to the scholarship:12/00257-9 - Molecular epidemiology and study of virulence factors of Staphylococcus aureus resistant to oxacillin isolated from wounds in patients treated at Basic Health Units in the city of Botucatu, BP.DR

Abstract

Due the dissemination of infections caused by MRSA, the need to create an effective vaccine against this pathogen. U.S. only clone USA300 causes 80% of CA-MRSA infections. In preliminary studies in the laboratory of the investigator Binh An Diep found that lipoproteins corresponding to 70% of the peptides found in the cell wall of USA300. Little is known about the role of the six most abundant lipoproteins found in the pathogenesis of S. aureus, and if they have a good standard for vaccine production. Thus, this study aims to determine the protective efficacy of immunization with recombinant lipoproteins in an experimental model of bacteremia. For the production of recombinant proteins will use the cloning and expression of lipoprotein using vector pTX15. 7 will groups be used of 15 A/J mice each group to be immunized with each of the six recombinant lipoproteins or placebo. Blood samples will be collected retro-orbital on days 0,11 and 20 for measuring whole antibody titer against each type of protein by ELISA. On day 22, the mice receive 5x107 CFU of wild strains USA300 and SF8300. After the mice will be monitored for seven days and euthanized, will be evaluated survival, bacteria counts, abscess formation and histopathology of the kidneys. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LINO PEREIRA-FRANCHI, ELIANE PATRICIA; NOGUEIRA BARREIRA, MARIA RACHEL; LIMA MOREIRA DA COSTA, NATALIA DE SOUSA; CASTELO BRANCO FORTALEZA, CARLOS MAGNO; RIBEIRO DE SOUZA DA CUNHA, MARIA DE LOURDES. Prevalence of and risk factors associated with the presence of Staphylococcus aureus in the chronic wounds of patients treated in primary health care settings in Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 50, n. 6, p. 833-838, . (11/10146-7, 13/10975-9, 12/00257-9)
LINO PEREIRA-FRANCHI, ELIANE PATRICIA; NOGUEIRA BARREIRA, MARIA RACHEL; LIMA MOREIRA DA COSTA, NATALIA DE SOUSA; MORAES RIBOLI, DANILO FLAVIO; ABRAAO, LIGIA MARIA; MARTINS, KATHERYNE BENINI; VICTORIA, CASSIANO; RIBEIRO DE SOUZA DA CUNHA, MARIA DE LOURDES. Molecular epidemiology of methicillin-resistant Staphylococcus aureus in the Brazilian primary health care system. TROPICAL MEDICINE & INTERNATIONAL HEALTH, v. 24, n. 3, p. 339-347, . (12/00257-9, 13/10975-9, 11/10146-7)

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