Comparative analysis between Elisa and radioimmunoassay for evaluation of plasmatic corticosterone in transport stress with Lithobates catesbeianus

Abstract

Front of the growing integration into Brazilian frogculture in nowadays, the practice of fattening in small and medium farms has been gaining space and with it the increased transport of juveniles of large producers to its members. The aim of this study is to evaluate the corticosterone in the froglet of bullfrog (Lithobates catesbeianus), submitted to the stress of transport according to the ELISA and radioimmunoassay. The animals will be purchased from a commercial frog farm in the froglet phase with average weight 15.0 to 20.0 g. The methodology will test two usual methods of transport used for commercial breeding frogs: transportation in plastic boxes and in PVC tubes. For both, froglet still be sampled in frog farm, soon after removal of the tanks (zero time). Then another 300 animals from the same batch will be packaged in plastic boxes (0,60 x0, 40 m) and in PVC tubes (1,5 x0, 10 m) with a density of 100 animals and will be transported by car lasting 4 hours. In the laboratory other animals will be sampled after arrival and sequentially sampled at times 0 ', 15', 30 ', 45', 60 ', 90' and 24 hours. The blood samples will be performed in the vessel hind limb. This blood will be centrifuged and the plasma obtained is analyzed by ELISA and radioimmunoassay. The diagnostic kits for both techniques are validated previously for the animal species studied. So this study was to evaluate the management of transport froglet usually used within the production. (AU)