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EFFECT OF CHRONIC SMOKING ON DNA GENE REPAIR METHYLATION IN ORAL MUCOSA.

Grant number: 13/02168-6
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): July 01, 2013
Effective date (End): June 30, 2015
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal researcher:Janete Dias Almeida
Grantee:Celina Faig Lima Carta
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Epigenetic changes, such as methylation, can provide genetic silencing that contributes to the development of oral squamous cell carcinoma. The present study aims to conduct a study of epigenetic alterations in the process of carcinogenesis in the oral mucosa, through a screening of methylation of genes repair from chronic smokers. Cytological material will be collected from patients of the Outpatient Treatment Program Smoking Heart Institute, Hospital das Clinicas, Faculty of Medicine, University of São Paulo (INCOR-HCFMUSP) and from outpatient discipline of Propedêutica Estomatológica, Institute of Science and Technology, UNESP- Univ Estadual Paulista, São José dos Campos- SP- Brazil. The sample will be studied in 2 groups as follow: Group 1: 10 smokers, who smoked more than 20 cigarrettes/day/10years and had no history of malignant oral neoplasm or clinical signs at the site evaluated, only male. Group 2: 10 nonsmokers equivalent in sex and age to group 1. Patients will be evaluated by history and the degree of nicotine dependence and expired carbon monoxide levels by Fagerstrom scale. Exfoliative cytology from the lateral border of the tongue will be performed. Smears will be evaluated for methylation profile analysis by PCR Array System, and validated by quantitative RT-PCR in real time for APX1, CCNH, hMLH1, hMSH2 and ATM genes. Statistical analysis will be conducted using analysis of variance (ANOVA), Kruskal-Wallis and Mann-Whitney test, with significance level of 5%.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LIMA, CELINA FAIG; OLIVEIRA ALVES, MONICA GHISLAINE; DO CARMO CARVALHO, BRUNA FERNANDES; DE LIMA, THAYNARA ALVES; COUTINHO-CAMILLO, CLAUDIA MALHEIROS; SOARES, FERNANDO AUGUSTO; SCHOLZ, JAQUELINE; ALMEIDA, JANETE DIAS. Is DNA ploidy related to smoking?. JOURNAL OF ORAL PATHOLOGY & MEDICINE, v. 46, n. 10, p. 961-966, . (12/05371-4, 13/09910-0, 13/02168-6)
LIMA CARTA, CELINA FAIG; OLIVEIRA ALVES, MONICA GHISLAINE; DE BARROS, PATRICIA PIMENTEL; CAMPOS, MARCIA SAMPAIO; SCHOLZ, JAQUELINE; CARDOSO JORGE, ANTONIO OLAVO; NUNES, FABIO DAUMAS; ALMEIDA, JANETE DIAS. Screening methylation of DNA repair genes in the oral mucosa of chronic smokers. ARCHIVES OF ORAL BIOLOGY, v. 92, p. 83-87, . (12/05371-4, 13/02168-6)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.