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Microbiological and LPS investigation and their antigenic activity in teeth with pulpal necrosis and irreversible pulpitis in pro-inflammatory cytokine production

Grant number: 13/02402-9
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2013
Effective date (End): November 30, 2016
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal researcher:Brenda Paula Figueiredo de Almeida Gomes
Grantee:Ariane Cássia Salustiano Marinho
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Associated scholarship(s):13/17928-6 - Analysis of Porphyromonas gingivalis lipopolysaccharide when presente in a bacterial comunity, BE.EP.DR


The knowledge of the microbial community involved in endodontic infections through molecular techniques and its inflammatory potential pulp and periradicular tissues are important for the understanding of the pathogenesis of diseases of endodontic origin and establishment of a more effective therapy. The objectives of this study are: 1) To investigate the presence of bacterial species targeted in cases of irreversible pulpitis and pulp necrosis by Nested-PCR; 2) To quantify the infectious load present in cases of irreversible pulpitis and pulp necrosis by Checkerboard DNA-DNA Hybridization; 3) To evaluate the effectiveness of the endodontic therapy using different auxiliary chemicals in the reduction of microbial load by Checkerboard DNA-DNA Hybridization technique; 4) To quantify LPS, through the Limulus Lysate, before (C1) and after (C2) the endodontic therapy, comparing "in vivo" the effectiveness of chemo-mechanical preparation (PQM) with 2.5% NaOCl, and Chlorhexidine 2% on its reduction; 5) To evaluate the inflammatory potential of endodontic content before (C1) and after the root canal preparation with 2.5% NaOCl and 2% Chlorhexidine (C2) in macrophage cell culture regarding the production of Pro-Inflammatory Cytokines - IL-1 ², TNF-± by the enzyme-linked immunosorbent assay (ELISA). Thirty patients will be selected for endodontic treatment due to irreversible pulpitis (n = 15) or Pulpal necrosis associated with periapical lesion (n = 15). The teeth will be divided into groups according to the auxiliary chemical used in PQM. Samples for evaluation of the microbiological content, levels of endotoxin and inflammatory potential will be collected with sterile non-pyrogenic/paper points before and after Endodontic therapy. The data will be tabulated and statistically analyzed by SPSS for Windows. (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARINHO, A. C. S.; TO, T. T.; DARVEAU, R. P.; GOMES, B. P. F. A. Detection and function of lipopolysaccharide and its purified lipid A after treatment with auxiliary chemical substances and calcium hydroxide dressings used in root canal treatment. International Endodontic Journal, v. 51, n. 10, p. 1118-1129, OCT 2018. Web of Science Citations: 4.
MARINHO, ARIANE C. S.; POLAY, ANA R. O.; GOMES, BRENDA P. F. A. Accuracy of Turbidimetric Limulus Amebocyte Lysate Assay for the Recovery of Endotoxin Interacted with Commonly Used Antimicrobial Agents of Endodontic Therapy. JOURNAL OF ENDODONTICS, v. 41, n. 10, p. 1653-1659, OCT 2015. Web of Science Citations: 6.
MARINHO, A. C. S.; MARTINHO, F. C.; GONCALVES, L. M.; RABANG, H. R. C.; GOMES, B. P. F. A. Does the Reciproc file remove root canal bacteria and endotoxins as effectively as multifile rotary systems?. International Endodontic Journal, v. 48, n. 6, p. 542-548, JUN 2015. Web of Science Citations: 14.
MARINHO, ARIANE C. S.; MARTINHO, FREDERIC C.; LEITE, FABIO R. M.; NASCIMENTO, GUSTAVO G.; GOMES, BRENDA P. F. A. Proinflammatory Activity of Primarily Infected Endodontic Content against Macrophages after Different Phases of the Root Canal Therapy. JOURNAL OF ENDODONTICS, v. 41, n. 6, p. 817-823, JUN 2015. Web of Science Citations: 5.
SALUSTIANO MARINHO, ARIANE CASSIA; MARTINHO, FREDERICO CANATO; ZAIA, ALEXANDRE AUGUSTO; RANDI FERRAZ, CAIO CEZAR; FIGUEIREDO DE ALMEIDA GOMES, BRENDA PAULA. Monitoring the effectiveness of root canal procedures on endotoxin levels found in teeth with chronic apical periodontitis. Journal of Applied Oral Science, v. 22, n. 6, p. 490-495, NOV-DEC 2014. Web of Science Citations: 10.
MARTINHO, FREDERIC C.; LEITE, FABIO R. M.; CHIESA, WANDERSON M. M.; NASCIMENTO, GUSTAVO G.; FERES, MAGDA; GOMES, BRENDA P. F. A. Signaling Pathways Activation by Primary Endodontic Infectious Contents and Production of Inflammatory Mediators. JOURNAL OF ENDODONTICS, v. 40, n. 4, p. 484-489, APR 2014. Web of Science Citations: 10.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
. Investigation of LPS pathobiology in endodotic infections and its susceptibility to different therapeutic modalities. 2016. 244 f. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba.

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