Association between single nucleotide polymorphism in genes of CRP, TNF-alfa and IL-10 and plasma fatty acids and their effect to a systemic inflammatory patter at a population-based study - ISA-Capital

Abstract

The nutritional transition is associated with the pattern of Western diets, which are characterized by low intake of cereals, fruits, vegetables and foods rich in omega-3 polyunsaturated fatty acids and monounsaturated fatty acid oleic (omega-9) and the high intake of red meat and food sources of saturated fatty acids, omega-6 polyunsaturated fatty acids and trans fatty acids. Both the Western diet and the intake of saturated and trans fatty acids are associated with increased inflammatory response, which, in turn, participates in the etiology of chronic diseases. The nutrigenetics aims to identify genetic variations that generate distinct metabolic responses to a specific pattern of food consumption in a particular population. Thus, genetic variations may interfere with the relationship between fatty acids intake and plasma inflammatory biomarkers levels. Thereby, the present study aims to investigate the association between single nucleotide polymorphisms related to C-reactive protein (CRP), tumor necrosis factor (TNF)-alpha, and interleukin (IL)-10 genes and lipid intake and its effects on plasma inflammatory biomarkers levels in a population-based study in the city of São Paulo. We will study 303 individuals, aged 20 to 59 years. The method used to collect dietary data was the 24-hour recall, applied in duplicate. The dietary data were transformed in energy and nutrients using Nutrition Data System for Research program (NDS-R, Minessota, USA). The domiciliary blood collection was performed by specialized professionals, after 12 hours fasting, with prior appointment. The plasma levels of IL-1 beta, IL-6, IL-8, IL-10, TNF-alpha, IL 12p70, adiponectin, CRP, macrophage chemotactic protein (MCP)-1, soluble intercellular adhesion molecule (sICAM)-1 and soluble vascular cell adhesion molecule (sVCAM)-1 will be determine through multiplex immunoassay technique. The genomic DNA will be extracted from whole blood using salting out method and genotyping of single nucleotide polymorphisms found in CRP (rs1205, rs1417938, rs1800947, rs2808630, rs3093059), TNF-alpha (rs1799964, rs1799724, rs1800629 and rs361525), and IL-10 (rs1800871, rs1800892 and rs1800872) genes will be performed by specific allele PCR technique.