Some biomarkers are bio-accumulators, for denouncing the presence of toxic substances. These consist of organisms that can survive in a narrow spectrum of natural conditions and environmental changes can cause changes in biological functions reflected in their genetic material. The mutagens are agents that cause alterations in the DNA sequence corresponding to the induced mutations. Being heavy metals the most common mutagens in aquatic environments. Baccharis trimera Less (gorse) is a shrubby plant that stands out for its ability to accumulate heavy metals present in the water. Equisetum giganteum L. (horsetail) is a vascular seedless plant considered a deposit of minerals and vitamins. The objective of this project is to assess the mutagenic potential of heavy metals and Bioaccumulative Baccharis trimera Less and Equisetum giganteum L. Specimens of gorse and horsetail are cultured and exposed to metal solution and controls for 30 days. The vegetative parts collected will be crushed and dried in an oven for the preparation of extracts. The ethanolic and aqueous extracts at different concentrations are prepared with ultrapure water. The heavy metals present in the extracts are quantified by atomic absorption analysis. The Allium cepa test will be conducted to analyze the genotoxic activity of extracts. The test Comet in root A. strain is performed in extracts to quantify DNA damage caused by the presence of mutagens (heavy metals). Daphnia magna is used as a bioindicator in toxicity test of the extracts.
News published in Agência FAPESP Newsletter about the scholarship: