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Bone Healing Following Mid-Femur Fracture in Diabetic Rats, with and without Insulin Treatment and Sodium Alendronate Therapy

Grant number: 13/02202-0
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): May 01, 2013
Effective date (End): February 28, 2014
Field of knowledge:Health Sciences - Medicine - Surgery
Principal Investigator:José Batista Volpon
Grantee:Ariane Zamarioli
Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Femoral bone tissue quality and post-fracture callus will be assessed in rats with diabetes induced by streptozotocin. Rats will be treated or not by insulin and sodium alendronate and randomly assessed to 8 groups: (1) diabetic rats; (2) diabetic rats under control by insulin; (3) control rats submitted to bone fracture; (4) diabetic rats submitted to bone fracture; (5) diabetic rats controlled by insulin and submitted to bone fracture; (6) control rats treated with insulin and sodium alendronate; (7) diabetic rats treated with insulin and sodium alendronate and (8) diabetic rats submitted to bone fracture and treated with insulin and sodium alendronate. In all groups, bones will be evaluated on days 14 and 28 post-fracture. Closed bone fracture will be created at the mid-femur with implant fixation inserted through the knee. Both bone callus and the non-fractured femur quality will be assessed using optical microscopy (tissue characterization), micro-tomography (qualitative and quantitative microstructure analysis), immunohistochemistry (bone formation and resorption evaluation: by means of dosage of osteocalcin, osteoprogeterin and RANK), bone mineral density analysis (DXA) and bone strength (mechanical test). Results will search the bone fracture and healing process, as well will evaluate the efficacy of blood level glucose control and sodium alendronate administration on the bone quality.

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