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PHYSICAL MAPPING AND MOLECULAR STRUCTURE OF B CHROMOSOMES IN THE GENUS Characidium ( CHARACIFORMES, CRENUCHIDAE )

Grant number: 13/02143-3
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2013
Effective date (End): December 31, 2016
Field of knowledge:Biological Sciences - Genetics - Animal Genetics
Principal researcher:Fausto Foresti
Grantee:Érica Alves Serrano Freitas
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated scholarship(s):15/01717-1 - Molecular structure of b chromosomes in the genus Characidium ( Characiformes, CRENUCHIDAE ), BE.EP.DR

Abstract

B chromosomes or supernumerary are genomic structures extra to karyotype pattern A and are present in several groups of eukaryotic organisms such as plants, fungi and animals. These chromosomes may have different origins, including derivation of the autosomes or from sex chromosomes and even being caused by interspecific crosses, which characterizes them as an interesting model for genetic and evolutionary studies. The advent of microdissection technique, which enables the direct isolation of DNA from any chromosomal region, has been providing advances in knowledge and new information about the structure and composition of these genomic elements in a significant number of organisms carrying B chromosomes. In this sense, it is intended to perform the cytogenetic analysis in molecular level of four species of genus Characidium, C. gomesi, C. oiticicai, C. pterostictum and C. alipioi which have B chromosomes in significant stages of differentiation, using the techniques of microdissection, amplification and comparison of the sequences allocated on the genome and the supernumerary chromosomes. The activities, which comprise the isolation of these elements by microdissection (DNAb), as well as genomic DNA extraction (DNAg) from individuals without chromosome B will be followed by mapping and PCR amplification of several repetitive DNA sequences in both types of DNA extracted (DNAg and DNAb), and certainly will result information to better understand the molecular structure of this DNA segment in the genome of these species, as well as clarify aspects of their origin.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SERRANO, ERICA A.; MELO, BRUNO F.; FREITAS-SOUZA, DIOGO; OLIVEIRA, MARIA L. M.; UTSUNOMIA, RICARDO; OLIVEIRA, CLAUDIO; FORESTI, FAUSTO. Species delimitation in Neotropical fishes of the genus Characidium (Teleostei, Characiformes). ZOOLOGICA SCRIPTA, v. 48, n. 1, p. 69-80, JAN 2019. Web of Science Citations: 3.
PANSONATO-ALVES, JOSE CARLOS; SERRANO, ERICA ALVES; UTSUNOMIA, RICARDO; CAMACHO, JUAN PEDRO M.; DA COSTA SILVA, GUILHERME JOSE; VICARI, MARCELO RICARDO; ARTONI, ROBERTO FERREIRA; OLIVEIRA, CLAUDIO; FORESTI, FAUSTO. Single Origin of Sex Chromosomes and Multiple Origins of B Chromosomes in Fish Genus Characidium. PLoS One, v. 9, n. 9 SEP 16 2014. Web of Science Citations: 17.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FREITAS, Érica Alves Serrano. Physical mapping and molecular structure of B chromosomes in species of the genus Characidium (Characiformes, Crenuchidae). 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.