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Staphylococcus spp. isolated from sub clinic mastitis in different Brazilian States: Antimicrobial resistance, detection of virulence factors and identification of clonal profiling

Grant number: 12/24135-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2013
Effective date (End): February 28, 2017
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal researcher:Maria de Lourdes Ribeiro de Souza da Cunha
Grantee:Priscila Luiza Mello
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


Mastitis is considered a great problem in dairy farming. Antimicrobials are often used to contribute to increase the selective pressure of microorganisms that are resistant to the main drugs. Animal World Health Organization aims to protect animal and human health against the risk of antimicrobial resistance from sick animals treatments.This study aims to identify and characterize clonal profile, virulence factors and antimicrobial resistance factors in Staphylococcus spp. isolated from cattle milk of animals with subclinical mastitis of diferent zones in Brasil. The samples will be obtained by "Embrapa Gado de Leite" from six diferent brazilian states including Paraná, Santa Catarina, Rio Grande do Sul, Minas Gerais, São Paulo and Pernambuco. This study includes all Staphylococcus spp. strains that shows to be oxacilin resistant by the disc diffusion method. This samples will be phenotypic and genotypic identified folowed by an investigation of enterotoxins and biofilm genes encoding presence. Thereafter, the study will include the determination of oxacilin resistance by identifying the mecA and homologue mecA genes (mecC) and the vancomycin resistance screening. Positive isolates to biofilm and toxin genes encoding presence will pass trought the qRT-PCR techinique to determinate these virulence factors expression. Furthermore the Staphylococcus spp. clonal profile will be investigated using the Pulsed Field Gel Electrophoresis (PFGE) technique and the Multilocus Sequence Typing (MLST) technique; this last one by typing the prevalent clones. Thus, the staphylococci species clonal profile isolated from chattle milk may contribute to understand the present strains standarts on different herds, allowing measures to prevent and control the spread of pathogenic and multiresistant staphylococci clones in different brazilian zones.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MELLO, PRISCILA LUIZA; MORAES RIBOLI, DANILO FLAVIO; MARTINS, LISIANE DE ALMEIDA; VASCONCELOS PAIVA BRITO, MARIA APARECIDA; VICTORIA, CASSIANO; ROMERO, LETICIA CALIXTO; RIBEIRO DE SOUZA DA CUNHA, MARIA DE LOURDES. Staphylococcus spp. Isolated from Bovine Subclinical Mastitis in Different Regions of Brazil: Molecular Typing and Biofilm Gene Expression Analysis by RT-qPCR. ANTIBIOTICS-BASEL, v. 9, n. 12 DEC 2020. Web of Science Citations: 0.
MELLO, PRISCILA LUIZA; PINHEIRO, LUIZA; MARTINS, LISIANE DE ALMEIDA; VASCONCELOS PAIVA BRITO, MARIA APARECIDA; RIBEIRO DE SOUZA DA CUNHA, MARIA DE LOURDES. beta-Lactam resistance and vancomycin heteroresistance in Staphylococcus spp. isolated from bovine subclinical mastitis. JOURNAL OF DAIRY SCIENCE, v. 100, n. 8, p. 6567-6571, AUG 2017. Web of Science Citations: 2.
MELLO, PRISCILA LUIZA; MORAES RIBOLI, DANILO FLAVIO; PINHEIRO, LUIZA; MARTINS, LISIANE DE ALMEIDA; VASCONCELOS PAIVA BRITO, MARIA APARECIDA; RIBEIRO DE SOUZA DA CUNHA, MARIA DE LOURDES. Detection of Enterotoxigenic Potential and Determination of Clonal Profile in Staphylococcus aureus and Coagulase-Negative Staphylococci Isolated from Bovine Subclinical Mastitis in Different Brazilian States. TOXINS, v. 8, n. 4 APR 2016. Web of Science Citations: 10.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MELLO, Priscila Luiza. Staphylococcus spp. isolados de mastite subclínica bovina em diferentes estados brasileiros: resistência antimicrobiana, detecção dos fatores de virulência e identificação do perfil clonal. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.

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