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Analysis of the dental discoloration with MTA and Portland cement using a bovine dental model

Grant number: 13/04054-8
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): October 05, 2013
Effective date (End): January 04, 2014
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:Marco Antonio Hungaro Duarte
Grantee:Marina Angélica Marciano da Silva
Supervisor: Josette Camilleri
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Research place: University of Malta (UoM), Malta  
Associated to the scholarship:11/13573-3 - Physicochemical and biological properties of experimental Portland-based sealer containing different radiopacifying agents, BP.DR


The aim of the study is to perform the analysis of microstructure and elements of cements and dentin/cement interface. The evaluated cements will be: I - White MTA, II - Portland cement (PC), III - PC with 20% of zirconium oxide (ZO), IV - PC with 50% of ZO, V - PC with 20% of calcium tungstate (CT) and VI - PC with 50% of CT. The analysis of microstructure will be performed using the energy dispersive X-ray (EDX) with the cements in powder form and after mixing with distilled water of 80% distilled water and 20% propylene glycol. To manipulate the cements a proportion of 1 g of powder and 0.3 mL of liquid will be used. For interfacial analysis, a total of 60 bovine teeth (n=10) will be used. The teeth will be sectioned in 10 x 10 mm enamel/dentin blocks. A cavity with 0.5 mm diameter and 1.5 mm depth will be performed in the center of the palatal portion of each specimen. The cavities will be filled with the cements and sealed with a composite resin. Then, the blocks will be immersed in distilled water. The color will be evaluated with a spectrophotometer in the periods of 1h, 24h, 15 days and 30 days after immersion in water. After 30 days, the blocks will be sectioned and analyzes using the EDX regarding the microstructure of cement/dentin interface. The results will be submitted to the normality test of D'Agostino and Person to verify the adequate statistical test (p < 0.05). (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARCIANO, MARINA ANGELICA; CAMILLERI, JOSETTE; LIA MONDELLI, RAFAEL FRANCISCO; AMOROSO, PABLO ANDRES; CAVENAGO, BRUNO CAVALINI; PEROCHENA, ALDO DEL CARPIO; HUNGARO DUARTE, MARCO ANTONIO. Potential tooth staining due to root canal sealers containing bismuth oxide and formaldehyde. ENDO-ENDODONTIC PRACTICE TODAY, v. 9, n. 1, p. 39-45, . (11/13573-3, 13/04054-8)
MARCIANO, MARINA ANGELICA; GUIMARAES, BRUNO MARTINI; AMOROSO-SILVA, PABLO; CAMILLERI, JOSETTE; HUNGARO DUARTE, MARCO ANTONIO. Physical and Chemical Properties and Subcutaneous Implantation of Mineral Trioxide Aggregate Mixed with Propylene Glycol. JOURNAL OF ENDODONTICS, v. 42, n. 3, p. 474-479, . (13/04054-8, 14/01003-6)
MARCIANO, MARINA ANGELICA; HUNGARO DUARTE, MARCO ANTONIO; CAMILLERI, JOSETTE. Calcium silicate-based sealers: Assessment of physicochemical properties, porosity and hydration. Dental Materials, v. 32, n. 2, p. E30-E40, . (13/04054-8, 11/13573-3)
MARCIANO, MARINA ANGELICA; HUNGARO DUARTE, MARCO ANTONIO; CAMILLERI, JOSETTE. Dental discoloration caused by bismuth oxide in MTA in the presence of sodium hypochlorite. CLINICAL ORAL INVESTIGATIONS, v. 19, n. 9, p. 2201-2209, . (13/04054-8, 11/13573-3)
MARCIANO, MARINA ANGELICA; COSTA, REGINALDO MENDONCA; CAMILLERI, JOSETTE; LIA MONDELLI, RAFAEL FRANCISCO; GUIMARAES, BRUNO MARTINI; HUNGARO DUARTE, MARCO ANTONIO. Assessment of Color Stability of White Mineral Trioxide Aggregate Angelus and Bismuth Oxide in Contact with Tooth Structure. JOURNAL OF ENDODONTICS, v. 40, n. 8, p. 1235-1240, . (13/04054-8, 12/06738-9, 11/13573-3)

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Filed patent(s) as a result of this research project

COMPOSIÇÃO ODONTOLÓGICA AGREGANTE E SEU PROCESSO DE PRODUÇÃO BR1020130213080 - Universidade de São Paulo (USP) . Marco Antonio Hungaro Duarte ; Marina Angelica Marciano da Silva - August 2013, 21