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Evaluation of the effects of aqueous extract of yerba mate (Ilex paraguariensis) during in vitro differentiation of mesenchymal stem cells from bone marrow of Wistar rats into osteogenic lineage

Grant number: 12/20547-1
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2013
Effective date (End): March 31, 2015
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Sandra Helena Penha de Oliveira
Grantee:Victor Gustavo Balera Brito
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


Osteoporosis is an osteometabolic disease mainly characterized by increased bone porosity with reduced bone mineral density. Epidemiologically significant, this pathology has shown the increasingly high prevalence in the world, a fact which can be explained by a higher life expectancy, based on this, osteoporosis has concerned public health entities worldwide. Current treatments can be divided into anabolic therapies, which aim to increase bone formation, or anti-resorptive therapies, which reduce bone matrix reabsorption. Studies have shown therapeutic properties in the consumption of yerba mate (Ilex paraguariensis St. Hil. var Paraguariensis), effects as hypolipidemic activity, weight reduction, and anti-inflammatory activity were emphasized. More recently, improvements in bone matrix density in women post-menopause were associated with yerba mate infusion consumption, however, in vitro studies in the literature describing the effects of Ilex paraguariensis in bone biology are still absent. As seen, the objective of this study is to assess the direct effect of soluble extract of Ilex paraguariensis during differentiation of mesenchymal stem cells from bone marrow stroma of young Wistar rats into the osteogenic lineage. For this purpose, different concentrations of the extract of Ilex paraguariensis will be added to the culture medium containing the classical osteogenic inducer (ascorbic acid, ²-glycerophosphate, and dexamethasone), which will be used for osteogenic differentiation assays where the following parameters will be assessed: cell proliferation, total protein, alkaline phosphatase, and mineralization. Furthermore, TNF-alpha dosage, a proinflammatory cytokine, will be performed to evaluate the possible immunomodulatory properties of the extract. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BALERA BRITO, VICTOR GUSTAVO; CHAVES-NETO, ANTONIO HERNANDES; DE BARROSA, THAMINE LANDIM; PENHA OLIVEIRA, SANDRA HELENA. Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells. Journal of Ethnopharmacology, v. 244, . (11/06070-5, 11/19458-1, 12/20547-1)

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