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Study of Actinocephalus divaricatus (Koern.)Sano (Eriocaulaceae) extracts by HPLC/PDA

Grant number: 13/01227-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2013
Effective date (End): December 31, 2013
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Organic Chemistry
Principal researcher:Lourdes Campaner dos Santos
Grantee:Ana Caroline Zanatta Silva
Home Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


Coupled techniques such as High-Performance Liquid Chromatography - Photodiode Array Detector (HPLC / PDA) has been effectively used in an attempt to establish the levels of the main species of secondary metabolites in plant extracts. The importance of this technique has also been demonstrated with the advance of technology, as new types of couplings have been developed as the coupling between liquid chromatography and mass spectrometry (High-Performance Liquid Chromatography-Mass Spectrometry, HPLC / MS), which allows analysis online various complex matrices. Mass spectrometry offers further possibilities how the coupling between two stages of analysis (MS / MS), this being one of the most used. Our research group has studied Eriocaulaceae species for over two decades, resulting in isolation and identification of a hundred molecules, unpublished some other existing literature with antioxidant properties, cytotoxic, antiulcer, anti-inflammatory, intestinal diseases, ulcerative colitis, and Crohn's disease. Thus, this family of plants has interesting chemistry consisting of biologically active molecules. Therefore, this project aims to study plant extracts belonging to Eriocaulaceae by coupled techniques such as HPLC / PDA, which will allow a qualitative and quantitative analysis quickly and effectively, enabling the identification of metabolites present in polar extracts of the species proposed for study in this project search. The evergreen plants are increasingly exploited commercially, both domestically and internationally. However, most are endemic to Brazil and some species are at serious risk of extinction. The difficulty in studying chemically extracts of these species is offered little mass for performing the step phytochemical and obtaining mass quantities necessary to carry out the biological assays and often even for identifying the molecule by spectroscopic methods. But studies of chemical species must be continued since there are still hundreds of species without studies. (AU)

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