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Effects of oxidative stress on spermatozoa and their relationship with embryonic development

Grant number: 12/22915-8
Support type:Scholarships in Brazil - Master
Effective date (Start): April 01, 2013
Effective date (End): November 30, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal researcher:Mayra Elena Ortiz D' Avila Assumpcao
Grantee:Letícia Signori de Castro
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Given the importance of sperm upon fertilization and early embryonic development, know the effects of changes resulting from cryopreservation, as the production of reactive oxygen species (ROS) and their impact on this cell is critical to the improvement of biotechnologies such as in vitro fertilization (IVF). The aim of this study will be evaluate the impact of different concentrations of agents that induce oxidative stress on sperm and the effect of oxidative stress in the early stages of embryo development. First, treated and control sperm will be stain with fluorescent probes and evaluate by flow cytometry capillary. Variables related to sperm chromatin structure (acridine orange), mitochondrial potential (JC-1), membrane integrity (FITC-PI) and sperm capacitation (chlortetraticlin), and some of then related to oxidative stress (dichlorfluorescein, CellROX®, and BODIPY-C11) will be analyze at this stage. Besides the microcapillary flow cytometry, part of the sediment will be separate for conducting the test of thiobarbituric acid reactive substances (TBARS). In a second phase, groups of sperm treated with agents that induce oxidative stress and control will be use in the production of in vitro embryos, looking for verify the impact of different levels of oxidative stress on in vitro fertilization and early embryonic development. For this, the embryos will be evaluate for cleavage and the rate of formation of pronuclei.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE CASTRO, LETICIA S.; DE ASSIS, PATRICIA M.; SIQUEIRA, ADRIANO F. P.; HAMILTON, THAIS R. S.; MENDES, CAMILLA M.; LOSANO, JOAO D. A.; NICHI, MARCILIO; VISINTIN, JOSE A.; ASSUMPCAO, MAYRA E. O. A.. Sperm Oxidative Stress Is Detrimental to Embryo Development: A Dose-Dependent Study Model and a New and More Sensitive Oxidative Status Evaluation. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, . (12/22915-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
CASTRO, Letícia Signori de. Effects of oxidative stress on spermatozoa and relationship with embryo development. 2014. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.

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