Previous studies have demonstrated that increased expression of inflammatory cytokines (TNF-±, IL-1² IL-6 e IL-8) is related to mucositis development. The overexpression of these molecules can also delay the repair process. Low-level laser therapy (LLLT) has showed positive clinical effects on oral mucositis lesions and in vitro studies have showed that LLLT can promote incease of cell metabolism and proliferation. However, there are no data regarding the effetcs of LLLT on cytokines involved on the ethiopathogenesis of mucositis. Thus, the aims of this project are to evaluate: the effect of these cytokines and LLLT on wound healing capacity of cultured fibroblastos (Study 1); the effect of LLLT on expression of inflammatory cytokines of cultured fibroblasts (Study 2); and the effect of inflammatory cytokines and LLLT on cell proliferation and factors involved on tissue repair using a 3D conective tissue and oral mucosa models (Study 3). Gingival fibroblasts will be maintained in ideal conditions for each methodology. LLLT will be delivered using a specific prototype LaserTABLE (InGaAsP - 780±3 nm of wavelength; 25 mW). After the specified treatments, viable cells, cell proliferation and migration, gene expression and protein expression will be determined. Data will be evaluated for normality and homocedasticity, and after selection of the approppriate statistical tests, a 5% significance level will be considered.
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