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Grant number: 12/17947-8
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): June 01, 2013
Effective date (End): December 31, 2015
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Josimeri Hebling Costa
Grantee:Fernanda Gonçalves Basso
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated scholarship(s):14/06057-7 - Development of a 3D oral mucosa model for in vitro studies, BE.EP.PD


Previous studies have demonstrated that increased expression of inflammatory cytokines (TNF-±, IL-1² IL-6 e IL-8) is related to mucositis development. The overexpression of these molecules can also delay the repair process. Low-level laser therapy (LLLT) has showed positive clinical effects on oral mucositis lesions and in vitro studies have showed that LLLT can promote incease of cell metabolism and proliferation. However, there are no data regarding the effetcs of LLLT on cytokines involved on the ethiopathogenesis of mucositis. Thus, the aims of this project are to evaluate: the effect of these cytokines and LLLT on wound healing capacity of cultured fibroblastos (Study 1); the effect of LLLT on expression of inflammatory cytokines of cultured fibroblasts (Study 2); and the effect of inflammatory cytokines and LLLT on cell proliferation and factors involved on tissue repair using a 3D conective tissue and oral mucosa models (Study 3). Gingival fibroblasts will be maintained in ideal conditions for each methodology. LLLT will be delivered using a specific prototype LaserTABLE (InGaAsP - 780±3 nm of wavelength; 25 mW). After the specified treatments, viable cells, cell proliferation and migration, gene expression and protein expression will be determined. Data will be evaluated for normality and homocedasticity, and after selection of the approppriate statistical tests, a 5% significance level will be considered.

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Scientific publications (11)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BASSO, FERNANDA G.; PANSANI, TAISA N.; MARCELO, CYNTIA L.; DE SOUZA COST, CARLOS ALBERTO; HEBLING, JOSIMERI; FEINBERG, STEPHEN E.. Phenotypic markers of oral keratinocytes seeded on two distinct 3D oral mucosa models. TOXICOLOGY IN VITRO, v. 51, p. 34-39, . (13/05879-0, 12/17947-8, 14/06057-7)
BASSO, FERNANDA GONCALVES; SILVEIRA TURRIONI, ANA PAULA; SOARES, DIANA GABIELA; BAGNATO, VANDERLEI SALVADOR; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Low-level laser therapy for osteonecrotic lesions: effects on osteoblasts treated with zoledronic acid. SUPPORTIVE CARE IN CANCER, v. 22, n. 10, p. 2741-2748, . (13/05879-0, 12/17947-8)
BASSO, FERNANDA G.; PANSANI, TAISA N.; SOARES, DIANA G.; SCHEFFEL, DEBORA L.; BAGNATO, VANDERLEI S.; DE SOUZA COSTA, CARLOS ALBERTO; HEBLING, JOSIMERI. Biomodulation of Inflammatory Cytokines Related to Oral Mucositis by Low-Level Laser Therapy. Photochemistry and Photobiology, v. 91, n. 4, p. 952-956, . (13/05879-0, 12/17947-8, 14/06057-7)
BASSO, FERNANDA GONCALVES; SOARES, DIANA GABRIELA; PANSANI, TAISA NOGUEIRA; SILVEIRA TURRIONI, ANA PAULA; SCHEFFEL, DEBORA LOPES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Response of a co-culture model of epithelial cells and gingival fibroblasts to zoledronic acid. Brazilian Oral Research, v. 30, n. 1, . (13/05879-0, 12/17947-8)
BASSO, F. G.; HEBLING, J.; MARCELO, C. L.; COSTA, C. A. DE SOUZA; FEINBERG, S. E.. Development of an oral mucosa equivalent using a porcine dermal matrix. BRITISH JOURNAL OF ORAL & MAXILLOFACIAL SURGERY, v. 55, n. 3, p. 308-311, . (12/17947-8, 14/06057-7, 13/05879-0)
BASSO, F. G.; TURRIONI, A. P. S.; ALMEIDA, L. F.; SOARES, D. G.; OLIVEIRA, C. F.; HEBLING, J.; DE SOUZA COSTA, C. A.. Nutritional deprivation and LPS exposure as feasible methods for induction of cellular - A methodology to validate for vitro photobiomodulation studies. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 159, p. 205-210, . (13/05879-0, 12/17947-8)
BASSO, FERNANDA G.; SOARES, DIANA G.; DE SOUZA COSTA, CARLOS ALBERTO; HEBLING, JOSIMERI. Low-level laser therapy in 3D cell culture model using gingival fibroblasts. Lasers in Medical Science, v. 31, n. 5, p. 973-978, . (13/05879-0, 12/17947-8)
BASSO, FERNANDA G.; PANSANI, TAISA N.; SOARES, DIANA G.; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. LLLT Effects on Oral Keratinocytes in an Organotypic 3D Model. Photochemistry and Photobiology, v. 94, n. 1, p. 190-194, . (14/06057-7, 13/05879-0, 12/17947-8)
BASSO, FERNANDA G.; PANSANI, TAISA N.; TURRIONI, ANA PAULA S.; SOARES, DIANA G.; DE SOUZA COSTA, CARLOS ALBERTO; HEBLING, JOSIMERI. Tumor Necrosis Factor-alpha and Interleukin (IL)-1 beta, IL-6, and IL-8 Impair In Vitro Migration and Induce Apoptosis of Gingival Fibroblasts and Epithelial Cells, Delaying Wound Healing. Journal of Periodontology, v. 87, n. 8, p. 990-996, . (13/05879-0, 12/17947-8)
BASSO, F. G.; SOARES, D. G.; PANSANI, T. N.; TURRIONI, A. P. S.; SCHEFFEL, D. L.; DE SOUZA COSTA, C. A.; HEBLING, J.. Effect of LPS treatment on the viability and chemokine synthesis by epithelial cells and gingival fibroblasts. ARCHIVES OF ORAL BIOLOGY, v. 60, n. 8, p. 1117-1121, . (13/05879-0, 12/17947-8)
BASSO, FERNANDA G.; SOARES, DIANA G.; PANSANI, TAISA N.; CARDOSO, LAIS M.; SCHEFFEL, DEBORA L.; DE SOUZA COSTA, CARLOS ALBERTO; HEBLING, JOSIMERI. Proliferation, Migration, and Expression of Oral-Mucosal-Healing-Related Genes by Oral Fibroblasts Receiving Low-Level Laser Therapy After Inflammatory Cytokines Challenge. Lasers in Surgery and Medicine, v. 48, n. 10, SI, p. 1006-1014, . (13/05879-0, 12/17947-8)

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