Evaluation of the effect of calcium rinse followed by use of fluoridated dentifrice on erosion and abrasion of bovine dentin in vitro

Abstract

This project will evaluate the effect of mouthwash with calcium before fluoride toothpaste on erosion associated to abrasion of bovine dentin in vitro. During 5 days, bovine dentin blocks (n = 20) will be submitted the erosion + abrasion. The study will include the following treatments: rinsing with calcium and fluoride dentifrice (BCa + DF), rinse with calcium and placebo dentifrice (BCa + DP), rinsing with deionized water and using fluoridated dentifrice (BP + DF), and rinsing with deionized water and dentifrice placebo (BP + DP). The erosion will be conducted 4 times per day for cola-type refrigerant (90 s each), for 5 days. For abrasion, the blocks will be brushed, using an electric toothbrush (Oral B-Pro Health Power) and the dentifrice slurry to be used for each group (3 g: 10 mL deionized water) for 15 s, 2x/day. Between the erosion and abrasion, each block will be immersed in 10 mL of deionized water or a solution of 150 mM calcium lactate for 1 min, with stirring, 2x/day. The response variable will be the analysis by profilometry (µm). The data will be checked for normality and homogeneity (Bartlett's test / Kolmogorov and Smirnov), and will apply the most appropriate statistical test results (p <0.05).Keywords: calcium, dental erosion, tooth abrasion, dentin, hydroxyapatite, remineralization, fluoride toothpaste.(AU)