Injectable bioactive cements functionalized with osteogenic peptide for bone regeneration

Abstract

Mesoporous materials have been widely studied because of their characteristics and potential applications. The mesoporous silica has high specific surface area (~ 1000 m2.g-1) and pore size usually around 2-30 nm attracting attention for applications in adsorption of drugs and proteins. The osteogenic growth peptide (OGP) is an endogenous tetradecapeptide, which generates active form covalent bonds with the proteinases, proving to be a good anabolic agent and hematopoietic stimulator. This project involves the continuation of studies on the results that resulted in the Master's of the student Larissa Mendes Souza. In this context, the aim of this work is the preparation of injectable bone cement with mesoporous silica and osteogenic peptide to aid in bone regeneration, along with their degradation and release of molecules of biological interest. The peptide is synthesized by solid phase method (SPPS, Fmoc strategy); purified and characterized by High Efficiency Liquid Chromatography (HPLC), mass spectrometry and amino acid analysis. The mesoporous materials will be characterized by X-ray Scattering at Low Angle (SAXS), nitrogen adsorption-desorption at 77K (BET) and Transmission Electron Microscopy (TEM). The cement will be analyzed for their physico-chemical characteristics such as cure time, mechanical strength, bioactivity in vitro and release of ions. The in vitro experiments are carried out to evaluate the cytotoxic and genotoxic potential of cement in cultured hamster ovary cells. The in vivo analysis will be performed using guided bone regeneration in critical bone defects in rat calvaria, which will be populated with the respective cements containing peptide adsorbed OGP, evaluation for biocompatibility and its osteoinductive properties in the presence of the peptide in osteogenesis .