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Injectable bioactive cements functionalized with osteogenic peptide for bone regeneration

Grant number: 12/21735-6
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2013
Effective date (End): February 29, 2016
Field of knowledge:Engineering - Biomedical Engineering - Bioengineering
Principal Investigator:Ana Maria Minarelli Gaspar
Grantee:Larissa Mendes de Lázari
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


Mesoporous materials have been widely studied because of their characteristics and potential applications. The mesoporous silica has high specific surface area (~ 1000 m2.g-1) and pore size usually around 2-30 nm attracting attention for applications in adsorption of drugs and proteins. The osteogenic growth peptide (OGP) is an endogenous tetradecapeptide, which generates active form covalent bonds with the proteinases, proving to be a good anabolic agent and hematopoietic stimulator. This project involves the continuation of studies on the results that resulted in the Master's of the student Larissa Mendes Souza. In this context, the aim of this work is the preparation of injectable bone cement with mesoporous silica and osteogenic peptide to aid in bone regeneration, along with their degradation and release of molecules of biological interest. The peptide is synthesized by solid phase method (SPPS, Fmoc strategy); purified and characterized by High Efficiency Liquid Chromatography (HPLC), mass spectrometry and amino acid analysis. The mesoporous materials will be characterized by X-ray Scattering at Low Angle (SAXS), nitrogen adsorption-desorption at 77K (BET) and Transmission Electron Microscopy (TEM). The cement will be analyzed for their physico-chemical characteristics such as cure time, mechanical strength, bioactivity in vitro and release of ions. The in vitro experiments are carried out to evaluate the cytotoxic and genotoxic potential of cement in cultured hamster ovary cells. The in vivo analysis will be performed using guided bone regeneration in critical bone defects in rat calvaria, which will be populated with the respective cements containing peptide adsorbed OGP, evaluation for biocompatibility and its osteoinductive properties in the presence of the peptide in osteogenesis .

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MENDES, L. S.; SASKA, S.; COELHO, F.; DE O CAPOTE, T. S.; SCAREL-CAMINAGA, R. M.; MARCHETTO, R.; CARRODEGUAS, R. G.; GASPAR, A. M. M.; RODRIGUEZ, M. A.. Injectable beta-TCP/MCPM cement associated with mesoporous silica for bone regeneration: characterization and toxicity evaluation. Biomedical Materials, v. 13, n. 2, . (12/21735-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LÁZARI, Larissa Mendes de. Injectables bioactives cements functionalized with osteogenic peptide for bone repair. 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Química. Araraquara Araraquara.

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