Effects of laser therapy on bone repair process in diabetic rats

Abstract

Diabetes Mellitus (DM) is a disease with systemic involvement, the bone tissue is drastically affected by hyperglycemia and the reduction and/or absence of insulin secretion. These metabolic changes diminish mineral density and bone, leaving the bone more susceptible to fractures. Accordingly, the process of bone repair is also reduced, increasing the chances of poor consolidation or non-union bone. Given this context, several features have been used in order to promote the bone repair process. Among these, low-level laser therapy (LLLT) has been highlighted due to analgesic, anti-inflammatory, and reparative. Although there are positive results on the application of LLLT in the tissue repair process, there is little evidence on the application of this therapeutic resource in diabetic bone tissue. Given the above, this study aims to determine the effects of laser therapy on the bone repair process in the tibiae of diabetic rats. Will be used 32 Wistar rats underwent the surgical procedure for realization of bone defects in tibias and were randomly divided into 2 groups: diabetic control and diabetic treated with LLLT (808 nm, 100 mW, 30 J / cm ²). These groups will still be divided into 2 subgroups consisting of 8 animals each, to perform the analysis on different dates of sacrifice. The subgroup will be sacrificed on the 15th day after surgery and Group B will be sacrificed on the 30th day after surgery. Will be performed histological, immunohistochemical, morphometric, and biomechanical. This work has the aim to contribute to filling gaps in the effects of laser therapy in diabetic bone tissue. (AU)