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Maintaining two root systems in biochemical and physiological aspects during plant growth and agronomic performance of Passiflora edulis F. flavicarpa grafted

Grant number: 12/16024-3
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): December 01, 2012
Effective date (End): February 01, 2015
Field of knowledge:Agronomical Sciences - Agronomy - Crop Science
Principal Investigator:Elizabeth Orika Ono
Grantee:William Hiroshi Suekane Takata
Host Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


About a third of the planted area of passion is located in the western state of São Paulo, however, the culture is impaired, leading to migration of culture to other regions due to the high level of infection by pathogens associated with the premature death of passion that is caused by soil-borne pathogens such as Fusarium oxysporym f. passiflorae Nectria haematococca and Phyophtora sp. Chemical control of these pathogens has been shown to be effective and an alternative to circumvent the problem of this disease is the use of the technique of grafting. The use of Passiflora giberti seems promising as a rootstock because, besides being resistant pathogens, has a high place with respect to their growth and development. Thus, the objective of this work is to study the grafting with the use of two systems radiciais compared to the conventional method of grafting and non-grafted plants on development. The evaluation of the effects of stress caused by both grafting methods provide the passion, changes in gas exchange and fluorométricas as well as productivity and quality of the fruits of Passiflora edulis f. flavicarpa. Will be conducted two experiments, the first being conducted to compare the addition of the root system of Passiflora giberti and the conventional method of grafting and the second experiment was to study the influence of grafting on yield and fruit quality of passion. Seeds of Passiflora edulis f. flavicarpa. and P. giberti be sown in polystyrene trays with 200 cells filled with commercial substrate pine bark. After 30 days, the seedlings will be transplanted into plastic bags filled with commercial substrate of pine and enriched with slow release fertilizer for six months, only one individual being planted per bag, with the exception of plants that possess two systems radicals. The two types of grafting (grafting and rootstocks multiple) of Passiflora edulis f. flavicarpa on P. giberti be carried out 30 days after transplantation. Analyses of enzyme activities will be conducted on samples taken in the region of grafting, 1 cm above and 1 cm below the site of grafting and non-grafted plants in the samples will be drawn from the same region in four collections of 3 in 3 days. The analysis of the development will be carried out by assessing the plant height, leaf number, leaf area, dry mass of roots and shoots, internode length, stem diameter, 5 cm below the bud, stem diameter at the time of grafting and stem diameter 5 cm above the bud. The evaluations of gas exchange will be made by measuring the rate of CO2 assimilation (A, mol CO2 m-2 s-1), transpiration rate (E, water vapor mmol m-2 s-1), conductance stomatal (gs, mol m-2 s-1) and intercellular CO2 concentration in the leaf (Ci, mmol CO2 mol-1 air). The photochemical activity will be analyzed by chlorophyll fluorescence. For the second experiment, the system will conduct a cordon at a spacing of 2.5 m between plants and 3.5 meters between rows. The evaluations will be conducted periodically production from November through June, evaluating fruit diameter, fruit length, fruit number, fruit weight and total fruit yield per plant. During the month of March will be held to evaluate the internal quality of fruits such as soluble solids, titratable acidity, soluble solids / titratable acidity (ratio), hydrogen potential (pH), vitamin C, firmness and sugar content reducers.

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
TAKATA, William Hiroshi Suekane. Two root systems maintenance in biochemical and physiological aspects during the plant development and agronomic performance of grafted passiflora edulis sims. 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agronômicas. Botucatu Botucatu.

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