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Development of molecularly imprinted extraction phase for analysis of parabens in plasma samples by liquid chromatography coupled to mass spectrometry sequence

Grant number: 12/21178-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): February 01, 2013
Effective date (End): January 31, 2015
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Maria Eugênia Queiroz Nassur
Grantee:Mariane Valéria Roldão
Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Parabens are neutral esters derived from p-hydroxybenzoic acid and at low concentrations (0.016 to 0.4%) they inhibit the development of microorganisms in cosmetic products. In the skin, the esterase enzyme hydrolyses the parabens present in cosmetics of topical use. However, incomplete hydrolysis may occur in the presence of large amounts of parabens, which may accumulate in the human body or even cause allergic dermatitis in sensitive individuals. Although regulatory organizations have authorized the use of parabens, more comprehensive studies are necessary to determine the true consequences of the potential damage caused by the daily exposure to parabens present in cosmetics. Biological fluids cannot be inserted into LC-MS/MS systems in their physiological state because of their complexity. They present interfering compounds, especially proteins, which can suppress the ionization of the analytes during ionization under pressure (API) (LC-MS analyses). As a consequence, the interferents elute with the analyses during the chromatographic separation or irreversibly adsorb onto the analytical column. This changes the analytic retention times, causes column death, and raises analysis costs. Solid Phase Extraction (SPE) is a liquid-solid separation technique that uses extraction devices in the solid phase. The complexity of plasma samples and the presence of parabens and the small amount in these matrices require the development of more selective SPE extraction phases to enhance the selectivity and specificity of the chromatographic method. Molecularly Imprinted Polymers (MIP) are synthetic materials with molecular recognition properties based on biomimetic systems that are similar to specific enzyme-substrate or antigen-antibody systems. This project aims to develop a molecularly imprinted phase extraction for the solid-phase extraction of parabens in plasma samples, for analyses in Liquid Chromatography coupled to tandem Mass Spectrometry.(AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARIANE V. ROLDÃO; LIDERVAN P. MELO; LUIS FELIPPE C. MIRANDA; MARCOS G. RESENDE; MARIA EUGÊNIA C. QUEIROZ. Development of Molecularly Imprinted Polymers for Solid Phase Extraction of Parabens in Plasma Samples and Analysis by UHPLC-MS/MS. Journal of the Brazilian Chemical Society, v. 28, n. 2, p. 257-265, . (12/21178-0)

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