One of the strategies used in the development of new drugs involves the discovery of compounds that modulate the enzymatic activity which is important in the infectious process of pathogenic agents. One interesting approach in the new ligands screening is the use of methods based in the enzyme immobilization in chromatographic supports coupling liquid chromatography systems multi and/or unidimensional. The use of IMERs (immobilized enzyme reactors) as stationary phase coupling high performance liquid chromatography is a fast and efficient screening method of compounds libraries or natural extracts. There are several advantages on using immobilized enzyme against the use of the enzyme in solution such as: easy separation of formed products, improvement in the enzyme stability and use of small amount of sample. The enzyme NTPDase2 of Leishmania favors the pathogen infection, it inhibits the host immune answer allowing a silent infection. The inhibitors screening for this enzyme can bring important alternatives for the treatment of leishmaniose and provide better life quality to affected populations by endemics diseases. In this study the NTPDase2 will be immobilized for the construction of ICERs (Immobilized Capillary Enzyme Reactors) that will be applied in the identification of active substances in synthetic and/or natural compounds libraries using chromatographic systems.
News published in Agência FAPESP Newsletter about the scholarship: