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Development of enzymatic model for deconstruction of sugarcane bagasse cell wall

Grant number: 12/18859-5
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): December 01, 2012
Effective date (End): March 22, 2014
Field of knowledge:Biological Sciences - Biochemistry - Enzymology
Principal Investigator:Fábio Márcio Squina
Grantee:Rosana Goldbeck Coelho
Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil
Associated research grant:08/58037-9 - Library generation for biomass-conversion enzymes from soil metagenome, AP.BIOEN.JP


The biodegradation of lignocellulosic materials has been the subject of many studies, because of the importance of the carbon cycle in nature, as well as its biotechnological potential for biofuels production. The conversion of lignocellulosic biomass into renewable fuels, such as bioethanol, has been considered a promising technology for the replacement fossil fuels and the expansion of ethanol production worldwide. An essential step in the conversion of lignocellulosic biomass into ethanol or other biorefinery products is the depolymerization of polysaccharides by enzymatic means. However, the high cost of the enzymes required for the plant cell wall degradation hinders the development of an economically viable lignocellulosic ethanol. On the face of it, by making of the use of the collection of enzymes belonging to the National Laboratory of Science and Technology Bioethanol (CTBE), we intend to develop a enzymatic model for the deconstruction of sugar cane bagasse cell wall (hydrothermally pretreated bagasse and sugar cane stalk), aiming at not only the better understanding of the role of enzymes on the plant biomass degradation, but also to map their accessibility and define which enzymes are essencial for the bioconversion. This study on biomass bioconversion will be usefull not only for sugar cane biomass, but also can became the starting point for studies using other lignocellulosic substrates such as eucalyptus, sorghum, grasses, and so. Another focus, which also add value on this project, it is glycomic profiling of the fractions generated after enzymatic hydrolisis of sugar cane cell wall. This glicomic study will provide the better understanding of enzyme mode of action, as well as, it will shed light on the enzymatic activities that are absent in the cocktail and are needed for the eficient hydrolysis of lignocellulosic material.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GOLDBECK, ROSANA; DAMASIO, ANDRE R. L.; GONCALVES, THIAGO A.; MACHADO, CARLA B.; PAIXAO, DOUGLAS A. A.; WOLF, LUCIA D.; MANDELLI, FERNANDA; ROCHA, GEORGE J. M.; RULLER, ROBERTO; SQUINA, FABIO M.. Development of hemicellulolytic enzyme mixtures for plant biomass deconstruction on target biotechnological applications. Applied Microbiology and Biotechnology, v. 98, n. 20, p. 8513-8525, . (13/18910-3, 08/58037-9, 12/18859-5)
GOLDBECK, ROSANA; GONCALVES, THIAGO A.; DAMASIO, ANDRE R. L.; BRENELLI, LIVIA B.; WOLF, LUCIA D.; PAIXAO, DOUGLAS A. A.; ROCHA, GEORGE J. M.; SQUINA, FABIO M.. Effect of hemicellulolytic enzymes to improve sugarcane bagasse saccharification and xylooligosaccharides production. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, v. 131, p. 36-46, . (12/20549-4, 12/18859-5)
MANDELLI, F.; BRENELLI, L. B.; ALMEIDA, R. F.; GOLDBECK, R.; WOLF, L. D.; HOFFMAM, Z. B.; RULLER, R.; ROCHA, G. J. M.; MERCADANTE, A. Z.; SQUINA, F. M.. Simultaneous production of xylooligosaccharides and antioxidant compounds from sugarcane bagasse via enzymatic hydrolysis. INDUSTRIAL CROPS AND PRODUCTS, v. 52, p. 770-775, . (13/03061-0, 08/58037-9, 11/14200-6, 12/18859-5)

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