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Effects of low level laser therapy associated to a BMP4 incorporated scaffold on dental pulp stem-cells differentiation and migration. An in vitro study.

Grant number: 12/16552-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2012
Effective date (End): August 31, 2015
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Marcia Martins Marques
Grantee:Ivana Marcia Alves Diniz
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):13/17268-6 - Effect of laser phototherapy on the transplantation of a BMP4-loaded hydrogel plus stem cells for dentin-pulp complex regeneration in vivo, BE.EP.DR


The maintenance of tooth vitality in the oral cavity is a great challenge of minimal invasive dentistry. Because of low remodeling ability of dental pulp tissue, particularly in mature teeth, conservative treatments of this tissue are prone to fail, especially when performed in consequence of carious process. Conventional endodontic treatment is based on total removal of dental pulp tissue and its substitution for synthetic materials, which turns tooth lifeless and brittle. Currently, no restorative material is able to totally mimic physical and chemical characteristics of health dental tissue. By the other side, cell therapy has been showing great potential to regenerate dentin-pulp complex. The amount of dentin and pulp tissue formed by tissue engineering associating dental pulp stem cells far exceeds the amount that would be generated in situ during the lifetime of an organism. Scaffolds, morphogenes and stem cells are the main components of tissue engineering researches and, recently, low level laser therapy (LLLT) has been considered a promising extra component, which is able to modulate cell functions for tissue regeneration. The objective of this study is to investigate the influence of LLLT combined to a poloxamer hydrogels scaffold with incorporation of BMP4 on the behavior of dental pulp stem cells. Cell adhesion, proliferation and migration in the biomaterial, as well as identification of proteins related to stem cell differentiation into odontoblasts will be evaluated using or not LLLT.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALVES DINIZ, IVANA MARCIA; HORTA, IVAY DINIZ; AZEVEDO, CYNTHIA SOARES; ELMADJIAN, THAIS REGINA; MATOS, ADRIANA BONA; LORENZETTI SIMIONATO, MARIA REGINA; MARQUES, MARCIA MARTINS. Antimicrobial photodynamic therapy: A promise candidate for caries lesions treatment. Photodiagnosis and Photodynamic Therapy, v. 12, n. 3, p. 511-518, . (12/16552-0, 13/03864-6)
DINIZ, IVANA M. A.; CARREIRA, ANA C. O.; SIPERT, CARLA R.; UEHARA, CINDI M.; MOREIRA, MARIA S. N.; FREIRE, LAILA; PELISSARI, CIBELE; KOSSUGUE, PATRICIA M.; DE ARAUJO, DANIELE R.; SOGAYAR, MARI C.; et al. Photobiomodulation of mesenchymal stem cells encapsulated in an injectable rhBMP4-loaded hydrogel directs hard tissue bioengineering. Journal of Cellular Physiology, v. 233, n. 6, p. 4907-4918, . (12/16552-0, 13/17268-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
DINIZ, Ivana Marcia Alves. Odonto/osteogenic differentiation of photomodulated mesenchymal stem cells in BMP4-loaded hydrogel. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) São Paulo.

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