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Varicocele effect and its treatment in proteomic and lipidomic pathways and in seminal oxidative stress in adults.

Grant number: 12/15039-7
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2012
Effective date (End): December 12, 2016
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Ricardo Pimenta Bertolla
Grantee:Mariana Camargo
Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

A varicocele is the main treatable cause of male infertility. Although present in 15% of the adult male population, 35% of infertile men and 80% of men with secondary infertility have a palpable varicocele. In addition, many studies associate varicocele to a decrease in semen quality, functional quality of spermatozoa, in testicular volume and proteomic composition of seminal plasma. The surgery (varicocelectomy) can improve sperm quality, but still do not know (i) which men should undergo surgery for varicocele and (ii) which molecular mechanisms determine this seminal improvement. A recent study from our group found that varicocelectomy leads to an early improvement (within 90 days) in seminal oxidative stress and in the biosynthesis of eicosanoids. We know therefore, that the correction of varicocele positively impacts the quality and functional expression of some proteins. We still need better map the proteome of seminal plasma and spermatozoa of these patients, because we do not know how it differs from semen of fertile men, neither can suggest which patients should or should not be operated.As a result, this project aims to study men with normal semen without varicocele (controls) and compare them with patients with varicocele before correction of varicocele and 90 and 360 days after subinguinal microsurgical varicocelectomy. In both groups (with and without varicocele) and in the three experimental periods (before, after 90 days and 360 days after varicocelectomy) will be performed an avaliation of sperm quality, functional sperm quality, the level of seminal lipid peroxidation and also a proteomic and lipidomic analysis of these patients.With these results, it is expected to get a better understanding of the mechanisms by which varicocelectomy can reestablish seminal homeostasis and the metabolic pathways present, as well as separate patients who will benefit in fact of the surgical intervention.

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CAMARGO, MARIANA; IBRAHIM, EMAD; ABALLA, TEODORO C.; CARDOZO, KARINA H. M.; CARVALHO, VALDEMIR M.; LYNNE, CHARLES M.; BRACKETT, NANCY L.; BERTOLLA, RICARDO P.. Proteomic insight of seminal plasma in spinal cord injured men submitted to oral probenecid treatment for improved motility. JOURNAL OF SPINAL CORD MEDICINE, . (12/15039-7, 14/17185-6, 17/14346-7)
CAMARGO, MARIANA; INTASQUI, PAULA; BERTOLLA, RICARDO PIMENTA. Proteomic profile of seminal plasma in adolescents and adults with treated and untreated varicocele. ASIAN JOURNAL OF ANDROLOGY, v. 18, n. 2, p. 194-201, . (14/11493-0, 14/17185-6, 12/15039-7)
DEL GIUDICE, P. T.; BELARDIN, L. B.; CAMARGO, M.; ZYLBERSZTEJN, D. S.; CARVALHO, V. M.; CARDOZO, K. H. M.; BERTOLLA, R. P.; CEDENHO, A. P.. Determination of testicular function in adolescents with varicocoele - a proteomics approach. ANDROLOGY, v. 4, n. 3, p. 447-455, . (12/15039-7, 14/17185-6, 14/11636-6)
CAMARGO, M.; INTASQUI, P.; BELARDIN, L. B.; ANTONIASSI, M. P.; CARDOZO, K. H. M.; CARVALHO, V. M.; FRAIETTA, R.; BERTOLLA, R. P.. Molecular pathways of varicocele and its repair - A paired labelled shotgun proteomics approach. JOURNAL OF PROTEOMICS, v. 196, p. 22-32, . (12/15039-7, 14/17185-6)
CAMARGO, MARIANA; IBRAHIM, EMAD; ABALLA, TEODORO C.; CARDOZO, KARINA H. M.; CARVALHO, VALDEMIR M.; LYNNE, CHARLES M.; BRACKETT, NANCY L.; BERTOLLA, RICARDO P.. Proteomic insight of seminal plasma in spinal cord injured men submitted to oral probenecid treatment for improved motility. JOURNAL OF SPINAL CORD MEDICINE, v. 44, n. 6, p. 966-971, . (12/15039-7, 14/17185-6, 17/14346-7)

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