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Extraction of organochlorine pesticides in fish tissues, employing acetone as solvent assisted by ultrasound bath

Grant number: 12/17884-6
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2012
Effective date (End): October 31, 2013
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal Investigator:Manoel Lima de Menezes
Grantee:Bruna Pastrello
Host Institution: Faculdade de Ciências (FC). Universidade Estadual Paulista (UNESP). Campus de Bauru. Bauru , SP, Brazil
Associated research project(s): 12/21093-4

Abstract

Considering that persistent organic pollutants (POPs) include synthetic substances belonging to different organic functions, including organochlorine pesticides (. Aldrin, Dieldrin, Endrin, Chlordane, Heptachlor, DDT, Toxaphene) and two industrial polychlorinated biphenyls (PCBs and hexachlorobenzene, which are the most persistent and bioaccumulative, with high toxicity to humans and animals. Accordingly, environmental and biological monitoring is necessary, in order to have greater control regarding the irresponsible use of these products. Though there are several analytical methodology reported in the literature to make determinations of this pesticide, they present some difficulties, requiring several steps to make the clean-up of the sample. The proposed project aims to optimize a new analytical method that allows performing the extraction of organochlorine pesticides in fish tissues, employing acetone as solvent assisted by ultrasound bath, making the method more quickly and not requiring further steps to purify the sample, since the use of acetone allows obtaining pesticide residues without impurities, which impair the determinations. (AU)

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