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Phytochemistry study in Blepharocalyx salicifolius Kuth O. Berg

Grant number: 12/17714-3
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2012
Effective date (End): June 30, 2014
Field of knowledge:Biological Sciences - Botany - Pant Physiology
Principal Investigator:Sonia Cristina Juliano Gualtieri
Grantee:Eduardo Habermann
Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil


Allelopathy can be defined as any process involving secondary metabolites produced by plants and microorganisms that influence the growth and development of natural and agricultural systems. When these substances are characterized it can be used in the prospecting of alternative products of medical-veterinary or agriculture interest. Therefore, the first step to be taken in phytochemical studies is the evaluation of activity of extracts of a plant species and their subsequent fractionation using the technique known as bioassay-guided isolation, which directs the isolation according to bioactivity evaluated in target species through coleoptiles bioassays, seed germination, seedling growth, control of fungal growth, among others. The present study aims to conduct the bioassay-guided isolation of crude extracts of bark and mature leaves of Blepharocalyx salicifolius Kuth O. Berg. For this study a series of chromatographic procedures will be adopted for the fractionation of crude extracts obtained, being directed by bioassays performed at each step, which will demonstrate what fractions are more promising towards the phytotoxic activity, fungicidal and/or antioxidant. The obtained data for the parameters evaluated in each bioassay (phytotoxicity, fungicide and antioxidant) will be before submitted to tests of normality (Shapiro-Wilk) and homogeneity (Levene), to be used in the appropriate statistical

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
E. HABERMANN; M. IMATOMI; F. C. PONTES; S. C. J. GUALTIERI. Atividade antioxidante e teor de polifenóis de extratos de cascas (súber), caule, folhas jovens e folhas maduras de Blepharocalyx salicifolius (Kunth) O. Berg (Murta). Brazilian Journal of Biology, v. 76, n. 4, p. 898-904, . (12/17714-3)
HABERMANN, EDUARDO; PEREIRA, VIVIANE DE CASSIA; IMATOMI, MARISTELA; PONTES, FLAVIA CEVITHEREZA; JULIANO GUALTIERI, SONIA CRISTINA. In vitro herbicide activity of crude and fractionated leaf extracts of Blepharocalyx salicifolius (Myrtaceae). BRAZILIAN JOURNAL OF BOTANY, v. 40, n. 1, p. 33-40, . (12/17714-3)
E. HABERMANN; F. C. PONTES; V. C. PEREIRA; M. IMATOMI; S. C. J. GUALTIERI. Potencial fitotóxico de folhas jovens de Blepharocalyx salicifolius (Kunth) O. Berg. Brazilian Journal of Biology, v. 76, n. 2, p. 531-538, . (12/17714-3)

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