Advanced search
Start date
Betweenand

Biosensors to detect Escherichia coli using the concept of an extended electronic tongue

Grant number: 12/15543-7
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): November 01, 2012
Effective date (End): October 31, 2017
Field of knowledge:Physical Sciences and Mathematics - Physics - Condensed Matter Physics
Principal Investigator:Osvaldo Novais de Oliveira Junior
Grantee:Flavio Makoto Shimizu
Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated research grant:13/14262-7 - Nanostructured films from biologically-relevant materials, AP.TEM
Associated scholarship(s):13/21076-5 - Sonochemical fabrication of microelectrode arrays for biosensors, BE.EP.PD

Abstract

This Project is aimed at developing biosensors to detect micro-organisms such as Escherichia coli, using the concept of an extended electronic tongue (e-tongue), in which the sensing units of the array comprise enzymes and polyelectrolytes in layer-by-layer (LbL) films. Novel film architectures will be developed with distinct polyelectrolytes and enzymes such as lysozyme, pepsin and tripsin, in order to seek synergy in the interactions among the components and the analytes. Film fabrication will be monitored with various experimental techniques, including UV-Vis absorption and fluorescence spectroscopies, FTIR spectroscopy and atomic force microscopy, which will also serve to characterize the film properties. The methods of detection will involve electrochemical techniques, e.g. voltammetry and amperometry, and electrical impedance spectroscopy, in measurements to be done in liquid samples in the static mode or in a flow injection analysis (FIA) system. Because we anticipate a large variability in the biological samples, the massive amounts of data generated in the electrochemical and electrical techniques will be analyzed with information visualization methods. This will allow a further step toward optimization of the sensitivity and selectivity of the biosensors. As for the scientific problem to be addressed, attempts will be made to correlate the film properties and data from the sensing experiments with the molecular-level interactions between film components and the analytes. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (27)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MATERON, ELSA M.; NASCIMENTO, GUSTAVO F.; SHIMIZU, FLAVIO M.; CAMARA, AMANDA S.; SANDRINO, BIANCA; FARIA, RONALDO C.; OLIVEIRA JR, OSVALDO N.. y Role of sphingomyelin on the interaction of the anticancer drug gemcitabine hydrochloride with cell membrane models. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 196, . (12/15543-7, 17/24053-7, 17/04712-6, 18/22214-6)
SOARES, ANDREY COATRINI; SOARES, JULIANA COATRINI; SHIMIZU, FLAVIO MAKOTO; RODRIGUES, VALQUIRIA DA CRUZ; AWAN, IRAM TAJ; MELENDEZ, MATIAS ELISEO; OLIVEIRA PIAZZETTA, MARIA HELENA; GOBBI, ANGELO LUIZ; REIS, RUI MANUEL; FREGNANI, JOSE HUMBERTO T. G.; et al. A simple architecture with self-assembled monolayers to build immunosensors for detecting the pancreatic cancer biomarker CA19-9. ANALYST, v. 143, n. 14, p. 3302-3308, . (13/14262-7, 12/15543-7)
MATERON, ELSA MARIA; MARCHETTO, REINALDO; ARAUJO, ANGELA REGINA; VEGA-CHACON, JAIME; PIVIDORI, I, MARIA; JAFELICCI JR, MIGUEL; SHIMIZU, FLAVIO M.; OLIVEIRA JR, OSVALDO N.; BOLDRIN ZANONI, MARIA VALNICE. A simple electrochemical method to monitor an azo dye reaction with a liver protein. Analytical Biochemistry, v. 553, p. 46-53, . (13/14262-7, 12/15543-7, 16/00991-5)
TEODORO, KELCILENE B. R.; SHIMIZU, FLAVIO M.; SCAGION, VANESSA P.; CORREA, DANIEL S.. Ternary nanocomposites based on cellulose nanowhiskers, silver nanoparticles and electrospun nanofibers: Use in an electronic tongue for heavy metal detection. SENSORS AND ACTUATORS B-CHEMICAL, v. 290, p. 387-395, . (17/12174-4, 12/15543-7, 14/21184-5)
MIYAZAKI, CELINA MASSUMI; SHIMIZU, FLAVIO MAKOTO; MEJIA-SALAZAR, J. R.; OLIVEIRA, OSVALDO N., JR.; FERREIRA, MARYSTELA. Surface plasmon resonance biosensor for enzymatic detection of small analytes. Nanotechnology, v. 28, n. 14, p. 6-pg., . (12/15543-7, 14/15093-7, 16/12311-9, 14/17519-1, 13/14262-7)
CARR, OLIVIA; RAYMUNDO-PEREIRA, PAULO A.; SHIMIZU, FLAVIO M.; SORROCHE, BRUNA PEREIRA; MELENDEZ, MATIAS ELISEO; PEDRO, RAFAEL DE OLIVEIRA; MIRANDA, PAULO B.; CARVALHO, ANDRE LOPES; REIS, RUI M.; ARANTES, LIDIA M. R. B.; et al. Genosensor made with a self-assembled monolayer matrix to detect MGMT gene methylation in head and neck cancer cell lines. Talanta, v. 210, p. 7-pg., . (15/12990-0, 12/15543-7, 18/02819-0, 13/14262-7, 16/01919-6, 19/13390-8, 17/03503-4)
ANDRE, RAFAELA S.; SHIMIZU, FLAVIO M.; MIYAZAKI, CELINA M.; RIUL, JR., ANTONIO; MANZANI, DANILO; RIBEIRO, SIDNEY J. L.; OLIVEIRA, JR., OSVALDO N.; MATTOSO, LUIZ H. C.; CORREA, DANIEL S.. Hybrid layer-by-layer (LbL) films of polyaniline, graphene oxide and zinc oxide to detect ammonia. SENSORS AND ACTUATORS B-CHEMICAL, v. 238, p. 795-801, . (14/16789-5, 13/14262-7, 12/23880-3, 12/15543-7)
DAIKUZONO, CRISTIANE M.; SHIMIZU, FLAVIO M.; MANZOLI, ALEXANDRA; RIUL, JR., ANTONIO; PIAZZETTA, MARIA H. O.; GOBBI, ANGELO L.; CORREA, DANIEL S.; PAULOVICH, FERNANDO V.; OLIVEIRA, JR., OSVALDO N.. Information Visualization and Feature Selection Methods Applied to Detect Gliadin in Gluten-Containing Foodstuff with a Microfluidic Electronic Tongue. ACS APPLIED MATERIALS & INTERFACES, v. 9, n. 23, p. 19646-19652, . (14/16789-5, 13/14262-7, 14/03691-7, 12/15543-7)
BRAUNGER, MARIA L.; SHIMIZU, FLAVIO M.; JIMENEZ, MAWIN J. M.; AMARAL, LUCAS R.; DE OLIVEIRA PIAZZETTA, MARIA H.; GOBBI, ANGELO L.; MAGALHAES, PAULO S. G.; RODRIGUES, VARLEI; OLIVEIRA, JR., OSVALDO N.; RIUL, JR., ANTONIO. Microfluidic Electronic Tongue Applied to Soil Analysis. CHEMOSENSORS, v. 5, n. 2, . (14/03691-7, 13/14262-7, 13/50942-2, 12/15543-7, 15/14836-9)
GRABOSKI, ADRIANA MARCIA; BALLEN, SANDRA CRISTINA; MANZOLI, ALEXANDRA; SHIMIZU, FLAVIO M.; ZAKRZEVSKI, CLAUDIO AUGUSTO; STEFFENS, JULIANA; STEFFENS, CLARICE. Array of Different Polyaniline-Based Sensors for Detection of Volatile Compounds in Gummy Candy. FOOD ANALYTICAL METHODS, v. 11, n. 1, p. 77-87, . (12/15543-7)
SOARES, JULIANA COATRINI; SOARES, ANDREY COATRINI; RAYMUNDO PEREIRA, PAULO AUGUSTO; RODRIGUES, VALQUIRIA DA CRUZ; SHIMIZU, FLAVIO MAKOTO; MELENDEZ, MATIAS ELISEO; NETO, CRISTOVAM SCAPULATEMPO; CARVALHO, ANDRE LOPES; LEITE, FABIO L.; MACHADO, SERGIO A. S.; et al. Adsorption according to the Langmuir-Freundlich model is the detection mechanism of the antigen p53 for early diagnosis of cancer. Physical Chemistry Chemical Physics, v. 18, n. 12, p. 8412-8418, . (13/14262-7, 12/17689-9, 12/15543-7)
GRABOSKI, ADRIANA MARCIA; BALLEN, SANDRA CRISTINA; GALVAGNI, ELISIANE; LAZZARI, THIAGO; MANZOLI, ALEXANDRA; SHIMIZU, FLAVIO MAKOTO; STEFFENS, JULIANA; STEFFENS, CLARICE. Aroma detection using a gas sensor array with different polyaniline films. ANALYTICAL METHODS, v. 11, n. 5, p. 654-660, . (12/15543-7)
GRACA, JULIANA SANTOS; MIYAZAKI, CELINA MASSUMI; SHIMIZU, FLAVIO MAKOTO; VOLPATI, DIOGO; MEJIA-SALAZAR, J. R.; OLIVEIRA, JR., OSVALDO N.; FERREIRA, MARYSTELA. On the importance of controlling film architecture in detecting prostate specific antigen. Applied Surface Science, v. 434, p. 1175-1182, . (14/15093-7, 13/14262-7, 16/12311-9, 12/15543-7, 14/17519-1, 13/23288-0)
AVILA, ORIANA I.; SANTOS, MOLIRIA V.; SHIMIZU, FLAVIO M.; ALMEIDA, GUSTAVO F. B.; SIQUEIR, JONATHAS P.; ANDRADE, MARCELO B.; BALOGH, DEBORA T.; RIBEIRO, SIDNEY J. L.; MENDONCA, CLEBER R.. Direct Femtosecond Laser Printing of PPV on Bacterial Cellulose-Based Paper for Flexible Organic Devices. MACROMOLECULAR MATERIALS AND ENGINEERING, v. 303, n. 10, . (16/11591-8, 12/15543-7, 13/20884-0)
MIYAZAKI, CELINA MASSUMI; SHIMIZU, FLAVIO MAKOTO; MEJIA-SALAZAR, J. R.; OLIVEIRA, JR., OSVALDO N.; FERREIRA, MARYSTELA. Surface plasmon resonance biosensor for enzymatic detection of small analytes. Nanotechnology, v. 28, n. 14, . (13/14262-7, 16/12311-9, 14/15093-7, 12/15543-7, 14/17519-1)
CARR, OLIVIA; RAYMUNDO-PEREIRA, PAULO A.; SHIMIZU, FLAVIO M.; SORROCHE, BRUNA PEREIRA; MELENDEZ, MATIAS ELISEO; PEDRO, RAFAEL DE OLIVEIRA; MIRANDA, PAULO B.; CARVALHO, ANDRE LOPES; REIS, RUI M.; ARANTES, LIDIA M. R. B.; et al. Genosensor made with a self-assembled monolayer matrix to detect MGMT gene methylation in head and neck cancer cell lines. Talanta, v. 210, . (17/03503-4, 12/15543-7, 18/02819-0, 15/12990-0, 13/14262-7, 19/13390-8, 16/01919-6)
SHIMIZU, FLAVIO M.; TODAO, FAGNER R.; GOBBI, ANGELO L.; OLIVEIRA, JR., OSVALDO N.; GARCIA, CARLOS D.; LIMA, RENATO S.. Functionalization-Free Microfluidic Electronic Tongue Based on a Single Response. ACS SENSORS, v. 2, n. 7, p. 1027-1034, . (14/24126-6, 13/14262-7, 12/15543-7)
JOSHI, NIRAV; DA SILVA, LUIS F.; JADHAV, HARSHARAJ S.; SHIMIZU, FLAVIO M.; SUMAN, PEDRO H.; M'PEKO, JEAN-CLAUDE; ORLANDI, MARCELO ORNAGHI; SEO, JEONG GIL; MASTELARO, VALMOR R.; OLIVEIRA, JR., OSVALDO N.. Yolk-shelled ZnCo2O4 microspheres: Surface properties and gas sensing application. SENSORS AND ACTUATORS B-CHEMICAL, v. 257, p. 906-915, . (13/14262-7, 16/23474-6, 14/23546-1, 12/15543-7, 13/07296-2)
MACHADO, JAISON C.; SHIMIZU, FLAVIO M.; ORTIZ, MANOEL; PINHATTI, MARINA S.; CARR, OLIVIA; GUTERRES, SILVIA S.; OLIVEIRA JR, OSVALDO N.; VOLPATO, NADIA M.. Efficient Praziquantel Encapsulation into Polymer Microcapsules and Taste Masking Evaluation Using an Electronic Tongue. BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, v. 91, n. 6, p. 865-874, . (13/14262-7, 12/15543-7)
RAYMUNDO-PEREIRA, PAULO A.; SHIMIZU, FLAVIO M.; COELHO, DYOVANI; PIAZZETA, MARIA H. O.; GOBBI, ANGELO L.; MACHADO, SERGIO A. S.; OLIVEIRA, JR., OSVALDO N.. A Nanostructured Bifunctional platform for Sensing of Glucose Biomarker in Artificial Saliva: Synergy in hybrid Pt/Au surfaces. BIOSENSORS & BIOELECTRONICS, v. 86, p. 369-376, . (13/14262-7, 12/17689-9, 12/15543-7)
MATERON, ELSA M.; SHIMIZU, FLAVIO M.; DOS SANTOS, KEVIN FIGUEIREDO; NASCIMENTO, GUSTAVO F.; GERALDO, VANANELIA P. N.; OLIVEIRA JR, OSVALDO N.; FARIA, RONALDO C.. Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance. BIOMEDICINE & PHARMACOTHERAPY, v. 145, . (12/15543-7, 17/24053-7, 16/00991-5, 18/22214-6)
SHIMIZU, FLAVIO M.; PASQUALETI, ANIELLI M.; TODAO, FAGNER R.; DE OLIVEIRA, JESSICA F. A.; VIEIRA, LUIS C. S.; GONCALVES, SUELY P. C.; DA SILVA, GABRIELA H.; CARDOSO, MATEUS B.; GOBBI, ANGELO L.; MARTINEZ, DIEGO S. T.; et al. Monitoring the Surface Chemistry of Functionalized Nanomaterials with a Microfluidic Electronic Tongue. ACS SENSORS, v. 3, n. 3, p. 716-726, . (13/14262-7, 15/25406-5, 13/22429-9, 12/15543-7, 14/24126-6)
JOSHI, NIRAV; DA SILVA, LUIS F.; SHIMIZU, FLAVIO M.; MASTELARO, VALMOR R.; M'PEKO, JEAN-CLAUDE; LIN, LIWEI; OLIVEIRA, JR., OSVALDO N.. UV-assisted chemiresistors made with gold-modified ZnO nanorods to detect ozone gas at room temperature. Microchimica Acta, v. 186, n. 7, . (13/14262-7, 16/23474-6, 14/23546-1, 12/15543-7, 13/07296-2, 17/12437-5)
DE LUCENA, NATHALIA C.; MIYAZAKI, CELINA M.; SHIMIZU, FLAVIO M.; CONSTANTINO, CARLOS J. L.; FERREIRA, MARYSTELA. Layer-by-layer composite film of nickel phthalocyanine and montmorillonite clay for synergistic effect on electrochemical detection of dopamine. Applied Surface Science, v. 436, p. 957-966, . (14/15093-7, 13/14262-7, 12/15543-7)
IBANEZ-REDIN, GISELA; FURUTA, ROBERTO H. M.; WILSON, DEIVY; SHIMIZU, FLAVIO M.; MATERON, ELSA M.; REBOLHO BATISTA ARANTES, LIDIA MARIA; MELENDEZ, MATIAS E.; CARVALHO, ANDRE L.; REIS, RUI MANUEL; CHAUR, MANUEL N.; et al. Screen-printed interdigitated electrodes modified with nanostructured carbon nano-onion films for detecting the cancer biomarker CA19-9. Materials Science & Engineering C-Materials for Biological Applications, v. 99, p. 1502-1508, . (17/12096-3, 13/14262-7, 15/01770-0, 16/00991-5, 12/15543-7)
BRAZ, DANIEL C.; NETO, MARIO POPOLIN; SHIMIZU, FLAVIO M.; SA, ACELINO C.; LIMA, RENATO S.; GOBBI, ANGELO L.; MELENDEZ, MATIAS E.; ARANTES, LIDIA M. R. B.; CARVALHO, ANDRE L.; PAULOVICH, FERNANDO, V; et al. Using machine learning and an electronic tongue for discriminating saliva samples from oral cavity cancer patients and healthy individuals. Talanta, v. 243, p. 8-pg., . (12/15543-7, 18/22214-6)
JOSHI, NIRAV; SHIMIZU, FLAVIO M.; AWAN, IRAM T.; M'PEKO, JEAN-CLAUDE; MASTELARO, VALMOR R.; OLIVEIRA, OSVALDO N., JR.; DA SILVA, LUIS F.; IEEE. Ozone sensing properties of nickel phthalocyanine:ZnO nanorod heterostructures. 2016 IEEE SENSORS, v. N/A, p. 3-pg., . (12/15543-7, 14/23546-1)

Please report errors in scientific publications list using this form.

Filed patent(s) as a result of this research project

GENOSSENSOR DE DNA PARA DETECÇÃO DO BIOMARCADOR MGMT E SEU USO PARA DIAGNÓSTICO DE CÂNCER ESPINOCELULAR DE CABEÇA E PESCOÇO BR1020200232878 - Fundação Pio XII (FP) ; Universidade de São Paulo (USP) . LIDIA MARIA REBOLHO BATISTA ARANTES / OSVALDO NOVAIS DE OLIVEIRA JUNIOR / ANDRÉ LOPES CARVALHO / BRUNA PEREIRA SORROCHE / FLÁVIO MAKOTO SHIMIZU / MATIAS ELISEO MELENDEZ / OLIVIA CARR / PAULO AUGUSTO RAYMUNDO PEREIRA / PAULO BARBEITAS MIRANDA / RAFAEL DE OLIVEIRA PEDRO / RUI MANUEL VIEIRA REIS - November 2020, 13