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Effect of disturbed environments on the scale epithelium and feeding rate of Prochilodus lineatus (Prochilodontidae)

Grant number: 12/08446-5
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2013
Effective date (End): February 28, 2015
Field of knowledge:Biological Sciences - Genetics - Mutagenesis
Principal researcher:Flavio Henrique Caetano
Grantee:Rebeca Mamede da Silva Alves
Home Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil


Due to the growing concern with global pollution it becomes more and more important to focus on assessing lethal and sublethal effects of toxic agents, using the most informative diagnostic techniques as possible. Furthermore the recent search is for non-invasive or non-lethal sampling techniques, which represent an atractive alternative to tradicional methods of assessing contamination in fish. Searching also for techniques that allow identification of toxic agent's effects on several levels of biological organization and grant a broad view of the consequences of exposure to contaminants. Keeping in mind that biodegradable detergents are one of the compounds most dumped into rivers, and that LAS (linear alkilbenzene sulphonate) represents one of the active principles of detergent most consumed on the market, the project whishes to evaluate morphological alterations (histological, histochemical and ultrastructural) on the scale epithelium of a fish species, Prochilodus lineatus, due to direct and constant exposure to two dilutions of LAS, compared to a control group. The study aims to associate such effects to alterations on the feeding behavior of the fish. The collected data will be analyzed using a Leica microscope with camera, a Phillips transmission electron microscope, a Hitachi TM3000 scanning electron microscope, and Image J software. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALVES, R. M. S.; PEREIRA, B. F.; RIBEIRO, R. G. L. G.; PITOL, D. L.; CIAMARRO, C. M.; VALIM, J. R. T.; CAETANO, F. H.. The scale epithelium as a novel, non-invasive tool for environmental assessment in fish: Testing exposure to linear alkylbenzene sulfonate. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 129, p. 43-50, . (12/03414-8, 12/08446-5)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ALVES, Rebeca Mamede da Silva. Efeito do ambiente alterado no epitélio da escama, nas brânquias e na alimentação de Prochilodus lineatus. 2015. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro Rio Claro.

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