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Clinical, hematological, biochemical and peritoneal fluid evaluation of horses submitted to percutaneous cecal puncture with special cecal puncture needle or trocar

Grant number: 12/11880-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): October 01, 2012
Effective date (End): March 31, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal researcher:Renata Gebara Sampaio Dória
Grantee:Gustavo Morandini Reginato
Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil


The bloat of the cecum in horses can be alleviated by the use of a long needle or trocar through the flank, in order to promote the release of gases. In most cases, the percutaneous cecal puncture is rarely used because of the risks of developing peritonitis. Twenty-two horses will be divided into three groups: Cecal Puncture Groups A and T (GA and GT), corresponding to sixteen animals submitted to percutaneous cecal puncture with a special cecal puncture needle (eight animals) and trocar (eight animals), respectively, and Control Group (CG), referring to the six animals not subjected to cecal puncture. Clinical evaluations will be performed daily and blood samples and peritoneal fluid in Cecal Puncture Groups (GA and GT) and Control Group (CG) will be collected and evaluated prior to initiation of the experiment (T0) and in two (T2), six (T6), 12 (T12), 24 (T24), 48 (T48), 72 (T72), 96 (T96) hours thereafter. After seven (D7) and 14 (D14) days, new samples of blood and peritoneal fluid will be collected in both groups. The blood examination will consist of hematological evaluation, serum biochemical analysis, and plasma acute-phase protein concentrations and the peritoneal fluid evaluation will involve visual, cytological, and biochemical analysis, which aim to determine changes that may characterize peritonitis in the studied horses. The purpose of this study is to comparatively evaluate the clinical, hematological, biochemical, plasma acute-phase protein concentrations and peritoneal fluid alterations after percutaneous cecal puncture using a special cecal puncture needle or trocar in horses and compare the results obtained from horses that did not undergo percutaneous cecal puncture, but who had suffered sequential sampling of peritoneal fluid. (AU)

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