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Effect of the association of proanthocyanidins and adhesives volatilization in the stability of the hybrid layer

Grant number: 12/08944-5
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): January 20, 2013
Effective date (End): January 19, 2014
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Giselle Maria Marchi
Grantee:Larissa Sgarbosa de Araújo Matuda
Supervisor: Ana Karina Bedran-Russo
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Research place: University of Illinois at Chicago (UIC), United States  
Associated to the scholarship:11/07984-0 - "effect of different temperatures of volatilization of solvents in adhesive systems on their degree of conversion and different wet bonding techniques on the stability of adhesive interface", BP.DR


The objective of this study is to evaluate the influence of proanthocyanidin (PA) and different temperatures to solvent volatilization of adhesive systems on the modulus of elasticity (ME) of the hybrid layer. The occlusal surfaces of the third molars human will be ground flat with no. 320 grit silicon carbide abrasive paper to remove part of the enamel (Buehler, Lake Bluff,IL). The teeth that will be used in this study will have their roots and the oclusal surfaces sectioned and discarded. Teeth will be sectioned into 0.5 mm thick beams (n=5 beams per tooth) in the mesio-distal direction. The slices will be randomly divided into 12 groups (n = 12 slices) according to the dentin treatment after demineralization (application of PA and control-without applicationof PA), adhesives containing different solvents [One-step (OS), Excite (EX) and Single Bond 2 (SB)] and temperature volatilization of adhesives (23 C; 40 degrees celsius). The exposed dentin will be demineralized in 10% phosphoric acid solution for 5 hours and then rinsed with water. In groups where PA will be applied, it will contact the dentin for 1 min and rinsed with distilled water. Adhesive systems (OS, EX, SB) will be applied according to the manufacturer's recommendations and with the group in question. After resin polymerization with Demetron 501, the slices will be stored in artificial saliva or in distilled water according to the group in question. For the mechanical testing (0.5 mm/min), the slices will be coupled to the mechanical testing machine (model V-1000 Vitrodyne, Chatillon, Greensboro, NC) being made immediate assessments of ME, after 1, 2, 3, 4 weeks and after 6 months on the same slice. The data obtained shall be submitted to the appropriate statistical analysis. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE ARAUJO MATUDA, LARISSA SGARBOSA; MARCHI, GISELLE MARIA; AGUIAR, THAIANE RODRIGUES; LEME, ARIENE ARCAS; AMBROSANO, GLAUCIA M. B.; BEDRAN-RUSSO, ANA KARINA. Dental adhesives and strategies for displacement of water/solvents from collagen fibrils. Dental Materials, v. 32, n. 6, p. 723-731, . (12/08944-5)

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