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Nanomechanical properties and micromechanical evaluation of dentin/adhesive interface after hydrolytic degradation: a nanoindentation and tridimensional finite element analyses

Grant number: 12/07012-1
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): April 20, 2012
Effective date (End): November 19, 2012
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Eduardo Passos Rocha
Grantee:Rodolfo Bruniera Anchieta
Supervisor: Paulo G. Coelho
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
Research place: New York University, United States  
Associated to the scholarship:10/12926-7 - Nanomechanical properties and micromechanical evaluation of dentin/adhesive interface after hydrolytic degradation: nanoidentation and tridimensional finite element analyses, BP.DR


OBJECTIVE: The goal of this study is to evaluate the behavior and mechanical properties of dentin/ adhesive interface (d/a) of specimens degraded in Hanks balanced salt solution (HBSS), after 24 hours of restorative procedures (T0) and after twelve months (T1) using nanoindentation and finite element analysis. MATERIAL AND METHODS: Five adhesive systems will be used (5 groups): SBMU: ScotchBond Multi-Purpose (Control), AEO - Adper Easy One; CSEB - Clearfil SE Bond; P90 - P90 Self etch adhesive; OUB - One-Up Bonf and for each adhesive system. 15 intact third molars will be used, 3 for each group (n=3). Each tooth will obtain at least 4 fragments of d/a interface in the mesio-distal direction. One specimen of each tooth will be used for SEM, with total of at least 3 specimen per group. After obtaining the specimen, it will be stored in HBSS. The SEM (JEOL - JMS 5400 Scanning Microscope, Tokyo, Japan) will be used to measure the d/a interface structures (hybrid layer, adhesive, dentin) dimensions and morphology, at 2 times: T0 - after 24 hours of restorative procedures; T1 - after 12 months of HBSS storage. RESULTS: The mean of the dimensions for each structure (hybrid layer, adhesive, dentin) will be obtained after the adhesive procedures for each group. The interfacial analysis and comparison will be assessed between groups and each time point of analysis. The descriptive statistical analysis will be performed to describe the results. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ANCHIETA, RODOLFO BRUNIERA; MACHADO, LUCAS SILVEIRA; MARTINI, ANA PAULA; DOS SANTOS, PAULO HENRIQUE; GIANNINI, MARCELO; JANAL, MALVIN; TOVAR, NICK; SUNDFELD, RENATO HERMAN; ROCHA, EDUARDO PASSOS; COELHO, PAULO G.. Effect of long-term storage on nanomechanical and morphological properties of dentin-adhesive interfaces. Dental Materials, v. 31, n. 2, p. 141-153, . (12/07012-1, 10/12926-7)
ANCHIETA, RODOLFO BRUNIERA; MACHADO, LUCAS SILVEIRA; SUNDFELD, RENATO HERMAN; REIS, ANDRE FIGUEIREDO; GIANNINI, MARCELO; LUERSEN, MARCO ANTONIO; JANAL, MALVIN; ROCHA, EDUARDO PASSOS; COELHO, PAULO G.. Effect of partially demineralized dentin beneath the hybrid layer on dentin-adhesive interface micromechanics. JOURNAL OF BIOMECHANICS, v. 48, n. 4, p. 701-707, . (12/07012-1, 10/12926-7)

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