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Standardization of methodology and analysis of unmetabolized folic acid after mandatory fortification with folic acid: population-based study in São Paulo City

Grant number: 12/05505-0
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): August 01, 2012
Effective date (End): July 31, 2013
Field of knowledge:Health Sciences - Nutrition - Nutritional Analysis of Population
Principal Investigator:Dirce Maria Lobo Marchioni
Grantee:Josiane Steluti
Supervisor: Jacob Selhub
Host Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Research place: Tufts University, United States  
Associated to the scholarship:10/19899-5 - Dietary intake and genetic variants of folate and its relation to folate, unmetabolized folic acid and homocysteine blood concentrations after mandoty folic acid fortification: population based study - ISA-Capital., BP.DR


Introduction: Many countries, including Brazil, fortification of foods with folic acid was adopted as a public strategy to prevent and folate deficiency. In the United States, post-fortification period, there was a positive evolution of nutritional parameters of folate, prevalence of inadequate intake falled, increased concentrations of vitamin and decreased homocysteine concentration. However, some individuals exceeded the limit maximum tolerable (UL), and had unmetabolized folic acid (UMFA) in the blood. The presence of UMFA is due the synthetic form intake. There are few studies about metabolism and biological effects of UMFA. However, the UMFA can be an important factor in safety issues associated with high intake of folic acid. Objective: To standardize an analytical methodology for detecting UMFA, analyze the UMFA concentrations in the population, and the relations with dietary intake, vitamins and homocysteine concentrations, and interaction with the genetic variants involved in folate metabolism. Methodology: This study was conducted in 750 individuals of the representative sample in São Paulo. Diet, anthropometric data and blood samples were collected. Biochemical analysis(folate, B6, B12 and homocysteine) DNA extraction and genotyping were performed. The UMFA will be determined by Kalmbach and Selhub"s methodology proposed. Multiple models will be done considering the results of dietary intake, blood concentrations and interaction with the genetic variants involved in folate metabolism. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
STELUTI, JOSIANE; REGINALDO, CHRISTINA; SELHUB, JACOB; PAUL, LIGI; FISBERG, REGINA MARA; MARCHIONI, DIRCE MARIA. Presence of circulating folic acid in plasma and its relation with dietary intake, vitamin B complex concentrations and genetic variants. EUROPEAN JOURNAL OF NUTRITION, v. 58, n. 8, p. 3069-3077, . (15/12196-2, 12/05505-0, 11/19788-1, 10/19899-5)
STELUTI, JOSIANE; PALCHETTI, CECILIA ZANIN; MIRANDA, ANDREIA MACHADO; FISBERG, REGINA MARA; MARCHIONI, DIRCE MARIA. DNA methylation and one-carbon metabolism related nutrients and polymorphisms: analysis after mandatory flour fortification with folic acid. BRITISH JOURNAL OF NUTRITION, v. 123, n. 1, p. 23-29, . (11/19788-1, 12/05505-0, 10/19899-5, 15/12196-2)
STELUTI, JOSIANE; MIRANDA, ANDREIA MACHADO; DE CARLI, EDUARDO; PALCHETTI, CECILIA ZANIN; FISBERG, REGINA MARA; LOBO MARCHIONI, DIRCE MARIA. Unmetabolized folic acid is associated with TNF-alpha, IL-1 beta and IL-12 concentrations in a population exposed to mandatory food fortification with folic acid: a cross-sectional population-based study in Sao Paulo, Brazil. EUROPEAN JOURNAL OF NUTRITION, v. 60, n. 2, . (10/19899-5, 12/05505-0, 11/19788-1, 15/12196-2)
STELUTI, JOSIANE; CARVALHO, ALINE M.; CARIOCA, ANTONIO A. F.; MIRANDA, ANDREIA; GATTAS, GILKA J. F.; FISBERG, REGINA M.; MARCHIONI, DIRCE M.. Genetic Variants Involved in One-Carbon Metabolism: Polymorphism Frequencies and Differences in Homocysteine Concentrations in the Folic Acid Fortification Era. NUTRIENTS, v. 9, n. 6, . (12/05505-0, 11/19788-1, 10/19899-5)

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