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LRP6 AND FRIZZLED6 EFFECTS IN THE ENDOTHELIAL DIFFERENTIATION OF DPSC

Grant number: 12/13039-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2012
Effective date (End): July 31, 2015
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:Carlos Henrique Ribeiro Camargo
Grantee:Gleyce Oliveira Silva
Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
Associated scholarship(s):12/24244-3 - The role of VEGF and dentin-derived factors in the differentiation of stem cells from human exfoliated teeth (shed) in odontoblasts and endothelial cells, BE.EP.DR

Abstract

The aim of this study is test the hypothesis that dental pulp stem cells (DPSC) differentiate into vascular endothelial cells through regulation of Wnt/B-catenin ligands LRP6 or Frizzled6. DPSC will be transduced with EGFP-tagged lentiviral sh-RNA vectors (LRP6, Frizzled6 or empty vector) for experiments. Frizzled6 and LRP6 effects will be evaluated by GSK-3beta and Beta-catenin expression, and by endothelial marks expression in the differentiation of DPSCs into endothelial vascular cells. To validate this results, transduced DPSC will be seed in tooth slices/scaffolds and implanted subcutaneously in immunosupressed mice. Cell presence in blood vessels will be determined by immunohistochemestry and HE staining.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVA, GLEYCE O.; ZHANG, ZHAOCHENG; CUCCO, CAROLINA; OH, MIN; CAMARGO, CARLOS H. R.; NOER, JACQUES E.. Lipoprotein Receptor-related Protein 6 Signaling is Necessary for Vasculogenic Differentiation of Human Dental Pulp Stem Cells. JOURNAL OF ENDODONTICS, v. 43, n. 9, p. 6-pg., . (12/13039-0, 12/24244-3)
SILVA, GLEYCE O.; ZHANG, ZHAOCHENG; CUCCO, CAROLINA; OH, MIN; CAMARGO, CARLOS H. R.; NOER, JACQUES E.. Lipoprotein Receptor-related Protein 6 Signaling is Necessary for Vasculogenic Differentiation of Human Dental Pulp Stem Cells. JOURNAL OF ENDODONTICS, v. 43, n. 9, S, p. S25-S30, . (12/13039-0, 12/24244-3)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Gleyce Oliveira. Efeitos do LRP6 e Frizzled6 na diferenciação endotelial de DPSC. 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. São José dos Campos São José dos Campos.

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