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RNA-Seq of bovine leukocytes from peripheral blood: a functional analysis of the transcriptome in cattle immunized with recombinant antigens from the cattle tick, Rhipicephalus microplus, and in infested cattle

Grant number: 12/15464-0
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): November 01, 2012
Effective date (End): October 31, 2013
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal researcher:Isabel Kinney Ferreira de Miranda Santos
Grantee:Sandra Regina Costa Maruyama
Supervisor abroad: Alvis Brazma
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Research place: European Bioinformatics Institute (EBI), England  
Associated to the scholarship:12/04087-0 - RNA-seq of bovine leukocytes from peripheral blood: a functional analysis of the transcriptome in cattle immunized with recombinant antigens from the cattle tick, Rhipicephalus microplus, and in infested cattle, BP.PD


The rational vaccine design depends on the understanding of immunological mechanisms stimulated by vaccination. The emerging field of Systems Vaccinology uses high-throughput data analysis (e.g. microarray and RNA-seq) for the identification of gene signatures that predict the immunogenicity of vaccines and consequently, the identification of potential mechanisms involved in vaccine efficacy. Due to variable efficacy of available anti-tick vaccine, the research group in ticks of FMRP-USP is screening new candidate antigens. So far, four recombinant salivary antigens have been tested as a multicomponent anti-tick vaccine in bovines, displaying 73.2% of efficacy. In order to obtain a global perspective of the molecular process trigged during immunization and infestation of bovines, the RNA from bovine leukocytes of the immunized and control groups was used to generate RNA-seq data (Illumina plataform). A total of 24 RNA-seq/Illumina libraries were obtained. The RNA-seq data have to be processed for the identification of differentially expressed genes, as well as the gene signatures related to protective immunity confered by the anti-tick vaccine and also related to the infestation. Thus, this BEPE application aims a training period in Bioinformatics at the Functional Genomic team of the European Bioinformatic Institute (EBI, Cambridge, UK). This training will be an excellent opportunity to explore the RNA-seq data using computational methods developed by EBI group. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GARCIA, GUSTAVO ROCHA; MARUYAMA, SANDRA REGINA; NELSON, KRISTINA T.; CHAVES RIBEIRO, JOSE MARCOS; GARDINASSI, LUIZ GUSTAVO; MENDES MAIA, ANTONIO AUGUSTO; FERREIRA, BEATRIZ ROSSETTI; KOOYMAN, FRANS N. J.; DE MIRANDA SANTOS, ISABEL K. F.. Immune recognition of salivary proteins from the cattle tick Rhipicephalus microplus differs according to the genotype of the bovine host. PARASITES & VECTORS, v. 10, . (12/15464-0, 12/04087-0, 13/00382-0)
MARUYAMA, SANDRA R.; GARCIA, GUSTAVO R.; TEIXEIRA, FELIPE R.; BRANDAO, LUCINDA G.; ANDERSON, JENNIFER M.; RIBEIRO, JOSE M. C.; VALENZUELA, JESUS G.; HORACKOVA, JANA; VERISSIMO, CECLIA J.; KATIKI, LUCIANA M.; et al. Mining a differential sialotranscriptome of Rhipicephalus microplus guides antigen discovery to formulate a vaccine that reduces tick infestations. PARASITES & VECTORS, v. 10, . (10/16464-8, 12/15464-0, 12/04087-0)
MARUYAMA, SANDRA R.; CARVALHO, BENILTON; GONZALEZ-PORTA, MAR; RUNG, JOHAN; BRAZMA, ALVIS; GARDINASSI, LUIZ GUSTAVO; FERREIRA, BEATRIZ R.; BANIN, TAMY M.; VERISSIMO, CECILIA J.; KATIKI, LUCIANA M.; et al. Blood transcriptome profile induced by an efficacious vaccine formulated with salivary antigens from cattle ticks. NPJ VACCINES, v. 4, n. 1, . (12/06708-2, 12/15464-0, 12/04087-0)

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