Gene therapy by RNA interference (RNAi) is a post-transcriptional silencing process that can suppress the expression of a particular gene. The RNA interference is a promising gene therapy approach for the treatment of many severe diseases that have no cure or well-defined treatments. However, the development of clinically appropriate, safe and effective delivery systems is necessary to enable this new therapy, since obstacles in the in vivo administration and distribution committed the clinical use of siRNAs (small interfering RNA). In addition, the topical delivery of siRNAs appears as a promising alternative for the treatment of cutaneous pathologies. In this context, this project aims the topical administration of liquid-crystalline nanodispersions, composed of monoolein, oleic acid and water, incorporated with adjuvants cationic polyethyleneimine and oleylamine, for carrier siRNA in the treatment of psoriasis with evaluation in vitro/in vivo of their activity and effectiveness, as well as investigation the mechanisms of cellular uptake of this delivery system.
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