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Biotechnology induction and cryopreservation of semen of Brycon cephalus (Günther, 1869) (Teleostei: Characidae)

Grant number: 12/11143-4
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2012
Effective date (End): March 31, 2014
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
Principal researcher:Alexandre Ninhaus Silveira
Grantee:Cristiane Bashiyo da Silva
Home Institution: Faculdade de Engenharia (FEIS). Universidade Estadual Paulista (UNESP). Campus de Ilha Solteira. Ilha Solteira , SP, Brazil


The quest for standardization and optimization of hormone levels is critical to the viability of fish semen, and one of the tools used for semen analysis is the evaluation of semen characteristics. The best semen quality is closely connected with the freezing of semen, semen because the better the better the results obtained after frozen semen. One of the problems that are currently for this method of freezing on the type of extender solution used in the technique, as this can cause injuries in the sperm cells, leading to deformation and viability of thawed sperm. Because of this, currently seeks to improve the cryopreservation technique for obtaining better results for the freezing of semen. For the technique of cryopreservation of fish semen is an effective way to improve the artificial breeding, genetic manipulation for facilitating the selection of breeding stock, allowing the establishment of gene banks, useful in the breeding programs and genetic conservation of endangered species extinction Therefore, this proposal aims at improving the hormonal induction technique for reproduction of Brycon cephalus, and develop methods of freezing and thawing to preserve the integrity and viability of sperm cells. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BASHIYO-SILVA, CRISTIANE; COSTA, RAPHAEL DA SILVA; RIBEIRO, DOUGLAS DE CASTRO; SENHORINI, JOSE AUGUSTO; VERISSIMO-SILVEIRA, ROSICLEIRE; NINHAUS-SILVEIRA, ALEXANDRE. Hormonal induction of Brycon cephalus (Characiformes, Characidae) to spermiation using D-ala6, pro9net-mGnRH + metoclopramide. ZYGOTE, v. 24, n. 3, p. 319-325, . (12/11143-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Cristiane Bashiyo da. Indução a espermiação e criopreservação espermática de Brycon cephalus (Gunther, 1869) (teleostei: characidae). 2014. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Engenharia. Ilha Solteira Ilha Solteira.

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