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Determination of aflatoxin B1 biomarkers and its applicability for evaluation of adsorbents efficiency in pigs

Grant number: 12/12705-6
Support Opportunities:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): August 01, 2012
Effective date (End): February 29, 2016
Field of knowledge:Agronomical Sciences - Food Science and Technology
Principal Investigator:Carlos Augusto Fernandes de Oliveira
Grantee:Mayra Carraro Di Gregorio
Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Associated research grant:10/20895-4 - Evaluation of AFB1-Lisin and AFB1-N7-guanin aducts as biomarkers of human and animal exposure to aflatoxins, AP.TEM
Associated scholarship(s):15/03900-8 - Determination of aflatoxin B1 metabolites in kidneys of pigs fed rations containing aflatoxin, antioxidants and adsorbent, alone or in combination, BE.EP.DD


Aflatoxins are carcinogenic compounds produced by fungi of the genus Aspergillus that contaminate food before and after processing, causing risks to human health and economic losses in animal production. Exposure to aflatoxin B1 (AFB1), the metabolite with greater toxicity produced by the fungus, occurs predominantly through ingestion of contaminated food, principally corn, peanuts and derivatives, whose contamination with AFB1 has been observed frequently in Brazil. The AFB1 is biotransformed by microsomal liver enzymes, resulting in the formation of adducts AFB1-N7-guanine and AFB1-lysine. The thematic project is structured in six sub-projects, which have independent and complementary objectives, in order to generate knowledge about the application of AFB1 adducts as biomarkers of exposure to aflatoxins in animals and humans, as well as its involvement in human hepatocellular carcinoma (HCC). The theme of the Sub-Project VI, to be executed in the work plan of the Direct Doctorate, is intended to verify the applicability of the determination of residues of AFB1 and AFB1-lysine in evaluating of adsorbents efficiency in pigs. Pigs will be evaluated for levels of productivity, serum biochemistry, histopathology, besides determination of residues of AFB1, AFB2a, AFM1, aflatoxicol and AFB1-lysine in serum and liver of pigs necropsied as biomarkers for the evaluation of adsorbents for aflatoxins in pigs. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DI GREGORIO, MAYRA C.; JAGER, ALESSANDRA V.; COSTA, ALINE A.; BORDIN, KELIANI; ROTTINHGHAUS, GEORGE E.; PETTA, TANIA; SOUTO, POLLYANA C. M. C.; BUDINO, FABIO E. L.; OLIVEIRA, CARLOS A. F.. Determination of Aflatoxin B-1-Lysine in Pig Serum and Plasma by Liquid Chromatography-Tandem Mass Spectrometry. JOURNAL OF ANALYTICAL TOXICOLOGY, v. 41, n. 3, p. 236-241, . (12/12705-6, 10/20895-4)
DI GREGORIO, MAYRA CARRARO; JAGER, ALESSANDRA VINCENZI; MAGGIO CASTRO SOUTO, POLLYANA CRISTINA; COSTA, ALINE ALVES; ROTTINGHAUS, GEORGE EDWIN; PASSARELLI, DANIELLE; LEMOS BUDINO, FABIO ENRIQUE; CORASSIN, CARLOS HUMBERTO; FERNANDES OLIVEIRA, CARLOS AUGUSTO. Determination of serum aflatoxin B-1-lysine to evaluate the efficacy of an aflatoxin-adsorbing feed additive in pigs fed an aflatoxin B-1-contaminated diet. MYCOTOXIN RESEARCH, v. 33, n. 2, p. 93-102, . (12/12705-6, 10/20895-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
GREGORIO, Mayra Carraro Di. Determination of aflatoxin B1 biomarkers and applicability for the evaluation of adsorbent\'s efficacy in pigs. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Zootecnica e Engenharia de Alimentos (FZE/BT) Pirassununga.

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