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Development of a disposable mimetic biosensor for organophosphorous and carbamate pesticides for the quality control of water supply

Grant number: 12/08750-6
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): August 01, 2012
Effective date (End): February 29, 2016
Field of knowledge:Engineering - Sanitary Engineering - Water Supply and Wastewater Treatment
Principal Investigator:Sergio Antonio Spinola Machado
Grantee:Lívia Flório Sgobbi
Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated scholarship(s):13/20701-3 - Development of an amperometric platform modified with graphene/quantum dots composite emitting at different wavelengths for pesticides detection, BE.EP.DR


With the introduction of synthetic pesticides in the early 70´s and with the subsequent awareness of their hazardous effects in the human health, analytical methods, presenting high sensitivity and selectivity as well as suitability for miniaturization aiming "in situ" applications on the field and in real time, have become the main objective of several research groups, around the world. However, portable and accurate devices, as required for such applications are mostly related with biosensors. Among them, the amperometric enzymatic biosensors are most appropriated. But the high cost of the enzyme used for pesticide monitoring (acetylcholinesterase) and its inherent instability have become their application a problematic issue. Moreover, some pesticides yield an irreversible inhibition in the enzymatic activity, making useless the biosensor, after the first measurement. In this way, cheap and disposable devices are of great interest. Previous work in the Grupo de Materiais Eletroquímicos e Métodos Eletroanalíticos from the Instituto de Química de São Carlos, USP, showed that the 4-[(1E)ethanehydrazonoyl]benzoic acid acts as a mimetic for acetilcholinesterase promoting the esterase of its substrate, acetylcholine being by the other hand, free from the issues of costs and instability observed in the enzyme itself. In this project, the utilization of 4-[(1E)ethanehydrazonoyl]benzoic acid is analyzed as an efficient mimetic for acetylcholinesterase in the esterase of acetylthiocholine chloride and its application in the development of disposable amperometric biosensor for the monitoring of carbamates and organophosphorous pesticides , as well as the detection and quantification of acetylcholine biomolecule. Structural modifications of the mimetic molecule will be investigated in order to optimize its mimetic activity and its proper immobilization on the transductor surface. (AU)

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Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SGOBBI, LIVIA F.; MACHADO, SERGIO A. S.. Functionalized polyacrylamide as an acetylcholinesterase-inspired biomimetic device for electrochemical sensing of organophosphorus pesticides. BIOSENSORS & BIOELECTRONICS, v. 100, p. 290-297, . (12/08750-6, 13/20701-3)
MORALES-NARVAEZ, EDEN; FLORIO SGOBBI, LIVIA; SPINOLA MACHADO, SERGIO ANTONIO; MERKOCI, ARBEN. Graphene-encapsulated materials: Synthesis, applications and trends. PROGRESS IN MATERIALS SCIENCE, v. 86, p. 1-24, . (12/08750-6, 13/17053-0, 13/20701-3)
CABRAL, MURILO F.; SGOBBI, LIVIA F.; KATAOKA, ERICA M.; MACHADO, SERGIO A. S.. On the behavior of acetylcholinesterase immobilized on carbon nanotubes in the presence of inhibitors. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 111, p. 30-35, . (11/10231-4, 12/08750-6)
SGOBBI, LIVIA F.; PINHO, VAGNER D.; CABRAL, MURILO F.; BURTOLOSO, ANTONIO C. B.; MACHADO, SERGIO A. S.. Hydrazone molecules as mimics for acetylcholinesterase. A new route towards disposable biosensors for pesticides?. SENSORS AND ACTUATORS B-CHEMICAL, v. 182, p. 211-216, . (12/08750-6, 08/09653-9)
SGOBBI, LIVIA F.; ZIBORDI-BESSE, LARISSA; RODRIGUES, BRUNO V. M.; RAZZINO, CLAUDIA A.; DA SILVA, JUAREZ L. F.; MACHADO, SERGIO A. S.. Polyhydroxamicalkanoate as a bioinspired acetylcholinesterase-based catalyst for acetylthiocholine hydrolysis and organophosphorus dephosphorylation: experimental studies and theoretical insights. CATALYSIS SCIENCE & TECHNOLOGY, v. 7, n. 15, p. 3388-3398, . (12/08750-6, 15/08523-8, 13/20701-3)
RAZZINO, CLAUDIA A.; SGOBBI, LIVIA F.; CANEVARI, THIAGO C.; CANCINO, JULIANA; MACHADO, SERGIO A. S.. Sensitive determination of carbendazim in orange juice by electrode modified with hybrid material. Food Chemistry, v. 170, p. 360-365, . (11/10231-4, 12/08750-6, 11/23047-7)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SGOBBI, Lívia Flório. Development of a disposable mimetic biosensor for organophosphorus and carbamate pesticides aiming quality control of water supply. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Química de São Carlos (IQSC/BT) São Carlos.

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