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Morphofunctional assessment and genomic culture of melanocytes from patients with facial melasma

Grant number: 12/05004-1
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2012
Effective date (End): January 31, 2016
Field of knowledge:Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
Principal researcher:Hélio Amante Miot
Grantee:Gabrielli Brianezi
Home Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


Melasma is a common disorder of hyperpigmentation, characterized by symmetric brown patches on skin exposed to sun. It's more frequent in the face, forehead and temples. Ultraviolet radiation (UVR), genetic influence and endocrine factors such as pregnancy, oral contraceptives, thyroid dysfunction and some medications and cosmetics are some of etiological factors. However none of them can be considered solely responsible for the development of the disease. The affected skin with melasma has some histological differences of healthy skin, such as increased melanogenic activity. The melasma pathogenesis is still unknown, while requiring a better characterization of the different behaviors of skin lesions and the adjacent, once they receive similar influences. The complexity of the skin, their intercellular interaction and presence of a small number of melanocytes per histologic section, hinder conclusions about what cell is the effector of disorder and their behavior. This study proposes a morphologic and functional evaluation of melanocytes primary culture from biopsies of patients with a facial melasma by transmission electron microscopy and genomics by PCR-array, and also the development a model that allows the investigation and determination of the effector cell of melasma, independently of other skin cells, allowing the progression of knowledge on melasma physiopathology. Additionally, we will investigate semiquantitatively the expression of peptides linked to melanogenesis in melasma (MC1-R, ±-MSH, COX-2, nuclear receptors of progesterone e estrogen-²) by double labeling immunofluorescence.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ESPOSITO, A. C. C.; BRIANEZI, G.; DE SOUZA, N. P.; MIOT, L. D. B.; MARQUES, M. E. A.; MIOT, H. A.. Exploring pathways for sustained melanogenesis in facial melasma: an immunofluorescence study. International Journal of Cosmetic Science, v. 40, n. 4, p. 420-424, . (12/05004-1, 12/09233-5)
CAVALCANTE ESPOSITO, ANA CLAUDIA; DE SOUZA, NATHALIA PEREIRA; BARTOLI MIOT, LUCIANE DONIDA; MIOT, HELIO AMANTE. Deficit in autophagy: A possible mechanism involved in melanocyte hyperfunction in melasma. INDIAN JOURNAL OF DERMATOLOGY VENEREOLOGY & LEPROLOGY, v. 87, n. 4, p. 585-586, . (12/09233-5, 12/05004-1)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BRIANEZI, Gabrielli. Activity evaluation of epidermo-melanin unit and dermal damage in melasma. 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina. Botucatu Botucatu.

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