Evaluation of microextraction technique to levetiracetam and risperidone determination by chromatographic techniques coupled with tandem mass spectrometry

Abstract

The procedure for sample preparation is crucial for the analysis of drugs present in biological fluids in order to carry out a prior sample preparation endogenous interferences and obtain a pre enriched fraction of the analyte in question. Conventional methods for the preparation of samples such as liquid-liquid extraction and solid phase extraction , are still the most used . However , miniaturized extraction techniques have been gaining ground in the analytical area due to its numerous advantages . These employ a very small amount of sample , a minimal amount or no organic solvents ( green chemistry ), potential automation , reduced in steps of sample preparation and speed. Thus, the objective of this project is to evaluate the dispersive liquid-liquid microextraction ( DLLME ) for review of levetiracetam ( LEV ), an antiepileptic, and analysis of risperidone ( RSP ) , an antipsychotic , in human plasma by chromatographic techniques spectrometry mass . Plasma Pretreatment to remove the plasma proteins is performed by ultrafiltration using Amicon filters ( pore size of 10 kDa . Then 1 ml of clear permeate which is subjected to DLLME parameters will be assessed extractor type and volume of solvent and dispersant , pH medium , effect of ionic strength and extraction time. DLLME After parameters have been optimized , the method will be validated in accordance with the official guides , ANVISA (2012 ) and EMEA (2011 ) and applied to a plasma sample patient under treatment.