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Glutathione S-transferase contribution in the Disperse Red 1 dye activation in Daphnia similis

Grant number: 12/01253-7
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2012
Effective date (End): December 31, 2012
Field of knowledge:Biological Sciences - Pharmacology - Toxicology
Principal researcher:Elisangela Franciscon Guimaro Dias
Grantee:Tsai Hsin Yu
Home Institution: Faculdade de Tecnologia (FT). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
Associated research grant:08/10449-7 - Assessment of occurrence, toxicity/genotoxicity and degradation processes of dyes in effluents and surface water, AP.TEM


Approximately 65% of the dyes used for coloring fabrics in the textile industry are azo dyes. These compounds are considered recalcitrant xenobiotic because of the presence of the de azo bond N = N, and they could be hazardous to the environment through the formation of mutagenic aromatic amines. The aim of this research is to study possible metabolites of Disperse Red 1 azo dye formed by juvenile microcrustacean Daphnia similis. Based on the experiments for the determination of glutathione s-transferase(GST)in Daphnia exposed to the dye, with and without the presence of enzyme inhibitors, it will be possible to clarify whether these enzymes are involved in the activation of these compounds. Analysis of the dye concentration that remained in the culture medium will be performed by spectrophotometer analysis. The possible metabolites formed by the exposed organisms will be analyzed by Q Trap LC-MS-MS. The results will be analyzed and discussed in order to understand whether GST activation is involved in the Disperse Red 1 toxicity. Furthermore, this technique can be used in the future as a biomarker of exposure to toxic substances activated by glutathione s-transferase and it can be one more sublethal endpoint to be measured in toxicity tests.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
YU, TSAI HSIN; DAFRE, ALCIR LUIZ; UMBUZEIRO, GISELA DE ARAGO; FRANCISCON, ELISANGELA. CYP-dependent induction of glutathione S-transferase in Daphnia similis exposed to a disperse azo dye. ECOTOXICOLOGY, v. 24, n. 1, p. 232-237, JAN 2015. Web of Science Citations: 5.

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