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Genetic diversity of Klebsiella pneumomiae producing KPC isolated from several hospitals in State of São Paulo

Grant number: 12/06828-8
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): October 01, 2012
Effective date (End): September 30, 2014
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal Investigator:Doroti de Oliveira Garcia
Grantee:Gabriela Rodrigues Francisco
Host Institution: Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

In 2005 a strain of Klebsiella pneumoniae producing KPC was first isolated in Brazil, quickly spreading through various hospitals in the State of Sao Paulo, as well as other states. The objective of this study is to evaluate the genetic diversity of strains of K. pneumoniae KPC-producing isolates from various hospitals in the state of Sao Paulo to detect clonal spread within and between hospitals. Approximately 100 strains of K. pneumoniae from several hospitals in the State of Sao Paulo, sent to the Adolfo Lutz Institute and previously confirmed in our laboratory as K. pneumoniae producing KPC by the use of antimicrobial susceptibility testing (disk diffusion and dilution) and PCR will be selected and subjected to pulsed field gel electrophoresis (PFGE) and Multi-Locus Sequence Typing (MLST). PFGE is made according to the protocol CDC modified Gautom (1997). Genomic DNA is digested with the restriction enzyme XbaI (Chef-III, BioRad) and the restriction profile will be analyzed by Bionumerics software, version 6.2, 2011 (Applied Maths, Kortrig, Belgium). For the seven MLST genes will be used "housekkeping" and the sequences of alleles and sequence type profiles (STs) will be verified by site http://pubmist.org/kpneumoniae. (AU)

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