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Standardized phytoterapics for the treatment of chronic diseases: Cytotoxic, mutagenic and protective properties and the modulation of gene expression

Grant number: 12/01996-0
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): June 01, 2012
Effective date (End): November 30, 2014
Field of knowledge:Health Sciences - Pharmacy - Toxicological Analysis
Principal researcher:Eliana Aparecida Varanda
Grantee:Juliana Mara Serpeloni
Home Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated research grant:09/52237-9 - Standardized herbal medicines for the treatment of chronic diseases, AP.BTA.TEM


The program "BIOTA/FAPESP" aims to identify and characterize the biodiversity of the State of São Paulo, defining the mechanisms for its conservation, economic potential and sustainable use. Some preliminary studies considering the biological activities of plant extracts were performed in the first stage of the project, however, their registration requires the assessment of herbal extracts standardized according to international standards, which is the objective of this second step. Thus, this project aims to collaborate in this important thematic project through in vitro evaluation of cytotoxic and mutagenic properties of standardized extracts from different plant species of the cerrado of São Paulo. Cell lines employed will be gastric cells ACP02 (tumoral) and primary culture of gastric cells (normal line), since the extracts evaluated in this study are used in treatment of gastrointestinal disorders and diseases. To assess cell viability and the mutagenic potential of the extracts, it will be used, respectively, the MTT assay and cytokinesis-block micronucleus test. The combination of dyes acridine orange and ethidium bromide, as well as flow cytometry, will be used to evaluate apoptosis. In parallel, the protective activity of these extracts will also be evaluated against the DNA damage and cytotoxicity induced by benzo(a)pyrene, a potent mutagen. The extracts that present protective activity in cells of the normal strain, but not in the tumoral cells will also be investigated by quantitative polymeric chain reaction array (RT-qPCR array).Expression of genes involved in oxidative stress, antioxidant defense process and inflammation will be investigate to propose the mechanisms of action of the protective effects of these extracts.Keywords: apoptosis, cytotoxicity, gastric cells ACP02; mutagenicity, phytotherapics, RT-qPCR array,

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SERPELONI, JULIANA MARA; SPECIAN, ANA FLAVIA LEAL; RIBEIRO, DIEGO LUIS; TUTTIS, KATIUSKA; HEREDIA-VIEIRA, SILVIA CRISTINA; VILEGAS, WAGNER; MARTINEZ-LOPEZ, WILNER; VARANDA, ELIANA APARECIDA; DE SYLLOS COLUS, ILCE MARA. Selective anticancer effects of Serjania marginata Casar. extract in gastric cells are mediated by antioxidant response. ENVIRONMENTAL TOXICOLOGY, v. 36, n. 8, p. 1544-1556, . (12/01996-0, 09/52237-9)
RIBEIRO, DIEGO LUIS; CILIAO, HELOISA LIZOTTI; LEAL SPECIAN, ANA FLAVIA; SERPELONI, JULIANA MARA; DE OLIVEIRA, MARCELO TEMPESTA; VARANDA, ELIANA APARECIDA; VILEGAS, WAGNER; SALDANHA, LUIZ LEONARDO; MARTINEZ-LOPEZ, WILNER; DOKKEDAL, ANNE LIGIA; et al. Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro. Cytotechnology, v. 70, n. 2, p. 713-728, . (09/52237-9, 12/01996-0, 09/16147-5)
SERPELONI, JULIANA MARA; LEAL SPECIAN, ANA FLAVIA; RIBEIRO, DIEGO LUIS; TUTTIS, KATIUSKA; VILEGAS, WAGNER; MARTINEZ-LOPEZ, WILNER; DOKKEDAL, ANNE LIGIA; SALDANHA, LUIZ LEONARDO; DE SYLLOS COLUS, ILCE MARA; VARANDA, ELIANA APARECIDA. Antimutagenicity and induction of antioxidant defense by flavonoid rich extract of Myrcia bella Cambess. in normal and tumor gastric cells. Journal of Ethnopharmacology, v. 176, p. 345-355, . (09/52237-9, 12/01996-0)
SERPELONI, J. M.; SPECIAN, A. F. L.; RIBEIRO, D. L.; BENICIO, L. M.; NUNES, H. L.; FRANCHI, L. P.; ROCHA, C. Q.; VILEGAS, W.; VARANDA, E. A.; COLUS, I. M. S.. Fridericia platyphylla (Cham.) LG Lohmann root extract exerts cytotoxic and antiproliferative effects on gastric tumor cells and downregulates BCL-XL, BIRC5, and MET genes. HUMAN & EXPERIMENTAL TOXICOLOGY, v. 39, n. 3, . (12/01996-0, 09/52237-9)

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